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著作: Nagae H./[中西 秀樹]/Urano Y./[荒瀬 誠治]/Serial Cultivation of Human Nail Matrix Cells under Serum-free Conditions/[The Journal of Dermatology]

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EID
243137
EOID
772140
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LastModified
2015年3月30日(月) 15:11:44
Operator
三木 ちひろ
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TRUE
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Owner
[副研究部長]/[徳島大学.大学院ヘルスバイオサイエンス研究部]
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Nagae H.
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 中西 秀樹
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Urano Y.
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 荒瀬 誠治
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Serial Cultivation of Human Nail Matrix Cells under Serum-free Conditions

副題 任意
要約 任意

(英) We have established serial cultures of human nail matrix cells (NMCs) under serum-free conditions. We cultured NMCs using two different methods depending upon the volume of nail matrix obtained. When a sufficient amount of nail matrix was obtained, they were minced and treated with 0.25% trypsin and 0.03% EDTA. The NMCs were transferred directly as a dispersed cell culture into KGM medium. Because a sufficient amount of matrix was rarely obtained, we developed a method by which NMCs were cultured primarily as implanted small matrices in Eagle's MEM (high Ca+ medium) supplemented with 15% fetal bovine serum for the first 4 to 5 days; during this time, the NMCs expanded from the matrices and formed colonies around them. NMCs then were cultured with KGM. In both methods, KGM medium supported the growth of NMCs without a biological feeder layer. These cells could be cultivated serially for at least seven passages. Half of the cells were positively stained with a monoclonal antibody against hair (hard) keratin which is expressed in nail matrix in vivo, indicating that the cells originated from the nail matrix. These methods will now permit investigations of nail matrix cells that previously were unfeasible because of the relative lack of cells and difficulties with propagation.

キーワード 推奨
  1. (英) Animals
  2. カルシウム(calcium)
  3. (英) Cattle
  4. (英) Cell Adhesion
  5. (英) Cell Count
  6. (英) Cell Culture Techniques
  7. 細胞分裂(cell division)
  8. (英) Cells, Cultured
  9. (英) Culture Media
  10. (英) Culture Media, Serum-Free
  11. (英) Edetic Acid
  12. (英) Fluorescent Antibody Technique, Indirect
  13. (英) Humans
  14. 免疫組織化学(immunohistochemistry)
  15. (英) Keratins
  16. (英) Nails
  17. トリプシン(trypsin)
発行所 推奨
誌名 必須 The Journal of Dermatology(日本皮膚科学会)
(pISSN: 0385-2407, eISSN: 1346-8138)
ISSN 任意 0385-2407
ISSN: 0385-2407 (pISSN: 0385-2407, eISSN: 1346-8138)
Title: The Journal of dermatology
Title(ISO): J Dermatol
Supplier: Japanese Dermatological Association
Publisher: Wiley Publishing
 (NLM Catalog  (医中誌Web  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 22
必須 8
必須 560 566
都市 任意
年月日 必須 1995年 8月 初日
URL 任意
DOI 任意
PMID 任意 7560452    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意 2-s2.0-0029095932
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.PublicationTypeList.PublicationType: Journal Article

  2. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't