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著作: [泰江 章博]/[米田 尚子]/[渡邉 哲平]/[田中 栄二]/Smad3リン酸化阻害が創傷治癒に及ぼす効果/国際歯科研究学会日本部会

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EID
237774
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608704
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2011年8月31日(水) 08:49:40
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泰江 章博
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泰江 章博
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種別 必須 国内講演発表
言語 必須 英語
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  1. 泰江 章博([徳島大学.病院.診療科.矯正歯科])
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  2. 米田 尚子([徳島大学.口腔科学教育部.口腔科学専攻.口腔健康科学講座.口腔顎顔面矯正学])
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  3. 渡邉 哲平([徳島大学.口腔科学教育部.口腔科学専攻.口腔健康科学講座.口腔顎顔面矯正学])
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  4. 田中 栄二([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.口腔顎顔面矯正学])
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(英) Effects of the inhibition of Smad3 phosphorylation on wound healing

(日) Smad3リン酸化阻害が創傷治癒に及ぼす効果

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(英) Objectives: The surgical closure of a cleft palate is considered to impair maxillary growth and dento-alveolar development. Transforming growth factor (TGF)-/Smad3 signaling is known to play an important role in wound healing. In our previous in vivo study, Smad3-deficient mice showed the accelerated re-epithelialization and tissue remodeling in the process of palatal wound repair with downregulation of TGF-1, monocyte chemotactic protein (MCP)-1 and macrophage inflammatory protein (MIP)-1 compared with wild-type mice. In this study, to develop a new remedy for palatal wound, we investigated the effect of a Specific Inhibitor of Smad3 (SIS3) which blocks TGF-1/Smad3 signaling through the inhibition of Smad3 phophorylation. Methods: The primary culture was performed for epithelial cells and fibroblasts from murine newborn mice-derived epidermis. The inhibition of Smad3 phosphorylation by SIS3 was verified with western blot analysis in fibroblasts. The effect of SIS3 on epithelial cell chemotaxis was assessed by in vitro migration assay. The SIS3 effect was also examined by three-dimensional collagen gel contraction assay, then -smooth muscle actin (SMA) expression was examined. Moreover, SIS3 was applied onto the mouse palate after creating mucoperiosteal wound. Histological analysis and immunohistochemistry were also performed for TGF-1, MIP-1 and MCP-1 expressions. Results: Smad3 phosphorylation was inhibited by SIS3 treatment in mice fibroblasts derived from newborn epidermis. The epithelial cells treated with SIS3 showed resistance to migration restraint by TGF-1 stimulation. Fibroblasts augmented contraction rate of collagen gels in the presence of TGF-1, whereas the lower ratio was observed in the case of SIS3 treatment and -SMA expression was decreased simultaneously. Scar formation in palate was reduced in the mice treated with SIS3 followed by the inhibitory effect of inflammation as seen the reduced expressions of TGF-1, MCP-1 and MIP-1. Conclusion: It was suggested that SIS3 treatment may be available for controlling wound repair by inhibiting TGF-1/Smad3 signaling.

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誌名 必須 (英) 58th JADR / (日) 国際歯科研究学会日本部会
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都市 必須 北九州(Kitakyushu/[日本国])
年月日 必須 2010年 11月 20日
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