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著作: Wójcik Cezary/[矢野 仁康]/DeMartino N. George/RNA interference of valosin-containing protein (VCP/p97) reveals multiple cellular roles linked to ubiquitin/proteasome-dependent proteolysis/[Journal of Cell Science]

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EID
236270
EOID
653649
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0
LastModified
2012年8月28日(火) 15:33:16
Operator
大家 隆弘
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Owner
[センター長]/[徳島大学.疾患酵素学研究センター]
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨 研究
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Wójcik Cezary
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 矢野 仁康
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) DeMartino N. George
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) RNA interference of valosin-containing protein (VCP/p97) reveals multiple cellular roles linked to ubiquitin/proteasome-dependent proteolysis

副題 任意
要約 任意

(英) We have used RNA interference (RNAi) to examine the functional relationship between valosin-containing protein (VCP/p97/Cdc48p/TER94) ATPase and the ubiquitin-proteasome system (UPS) in Drosophila S2 and human HeLa cells. In both cell types, RNAi of VCP (and, to a lesser extent, of certain VCP-interacting proteins) caused significant accumulation of high-molecular-weight conjugates of ubiquitin, an indication of inhibited UPS function. However, decreased VCP levels did not directly inhibit proteasome activity. In HeLa cells, polyubiquitinated proteins accumulated as dispersed aggregates rather than as single aggresomes, even in the presence of proteasome inhibitors, which normally promote aggresome formation. RNAi of VCP caused extensive vacuolization of the cytoplasm, and proteasome inhibitors exaggerated this feature. RNAi of VCP had little effect on S2 cell proliferation but blocked cell-cycle progression and induced mitotic abnormalities and apoptosis in HeLa cells. These results indicate that VCP plays an important general role in mediating the function of the UPS, probably by interacting with potential proteasome substrates before they are degraded by the proteasome.

キーワード 推奨
  1. (英) Adenosine Triphosphatases
  2. (英) Animals
  3. アポトーシス(apoptosis)
  4. (英) Caspases
  5. 細胞周期(cell cycle)
  6. (英) Cell Cycle Proteins
  7. 細胞分裂(cell division)
  8. (英) Cells, Cultured
  9. (英) Cysteine Endopeptidases
  10. (英) Drosophila
  11. (英) Flow Cytometry
  12. (英) HeLa Cells
  13. (英) Humans
  14. (英) Microscopy, Fluorescence
  15. (英) Multienzyme Complexes
  16. (英) Proteasome Endopeptidase Complex
  17. (英) RNA, Small Interfering
  18. (英) Ubiquitin
発行所 推奨
誌名 必須 Journal of Cell Science([The Company of Biologists Limited])
(pISSN: 0021-9533, eISSN: 1477-9137)
ISSN 任意 0021-9533
ISSN: 0021-9533 (pISSN: 0021-9533, eISSN: 1477-9137)
Title: Journal of cell science
Title(ISO): J Cell Sci
Publisher: Company of Biologists
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 117
必須 2
必須 281 292
都市 任意
年月日 必須 2003年 12月 2日
URL 任意
DOI 任意 10.1242/jcs.00841    (→Scopusで検索)
PMID 任意 14657277    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9040, USA.

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  4. (英) Article.PublicationTypeList.PublicationType: Research Support, U.S. Gov't, P.H.S.