233697: Haraguchi Kyoko/Suzuki Takahiro/Koyama Noriko/Kumano Keiki/Nakahara Fumio/Matsumoto Akihiko/Yokoyama Yasuhisa/Sakata-Yanagimoto Mamiko/Masuda Shigeo/Takahashi Tsuyoshi/Kamijo Aki/Takahashi Koki/Takanashi Minoko/Okuyama Yoshiki/[安友康二]/Sakano Seiji/Yagita Hideo/Kurokawa Mineo/Ogawa Seishi/Chiba Shigeru/Notch activation induces the generation of functional NK cells from human cord blood CD34-positive cells devoid of IL-15./[The Journal of Immunology]

著作: Haraguchi Kyoko/Suzuki Takahiro/Koyama Noriko/Kumano Keiki/Nakahara Fumio/Matsumoto Akihiko/Yokoyama Yasuhisa/Sakata-Yanagimoto Mamiko/Masuda Shigeo/Takahashi Tsuyoshi/Kamijo Aki/Takahashi Koki/Takanashi Minoko/Okuyama Yoshiki/[安友康二]/Sakano Seiji/Yagita Hideo/Kurokawa Mineo/Ogawa Seishi/Chiba Shigeru/Notch activation induces the generation of functional NK cells from human cord blood CD34-positive cells devoid of IL-15./[The Journal of Immunology]

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種別

必須

学術論文(審査論文)

言語

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英語

招待

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審査

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カテゴリ

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共著種別

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学究種別

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組織

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著者

必須

(英) Haraguchi Kyoko

役割任意

貢献度任意

学籍番号推奨
(英) Suzuki Takahiro

役割任意

貢献度任意

学籍番号推奨
(英) Koyama Noriko

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貢献度任意

学籍番号推奨
(英) Kumano Keiki

役割任意

貢献度任意

学籍番号推奨
(英) Nakahara Fumio

役割任意

貢献度任意

学籍番号推奨
(英) Matsumoto Akihiko

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貢献度任意

学籍番号推奨
(英) Yokoyama Yasuhisa

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貢献度任意

学籍番号推奨
(英) Sakata-Yanagimoto Mamiko

役割任意

貢献度任意

学籍番号推奨
(英) Masuda Shigeo

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貢献度任意

学籍番号推奨
(英) Takahashi Tsuyoshi

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貢献度任意

学籍番号推奨
(英) Kamijo Aki

役割任意

貢献度任意

学籍番号推奨
(英) Takahashi Koki

役割任意

貢献度任意

学籍番号推奨
(英) Takanashi Minoko

役割任意

貢献度任意

学籍番号推奨
(英) Okuyama Yoshiki

役割任意

貢献度任意

学籍番号推奨
安友康二([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.病理系.生体防御医学])

役割任意

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学籍番号推奨
(英) Sakano Seiji

役割任意

貢献度任意

学籍番号推奨
(英) Yagita Hideo

役割任意

貢献度任意

学籍番号推奨
(英) Kurokawa Mineo

役割任意

貢献度任意

学籍番号推奨
(英) Ogawa Seishi

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学籍番号推奨
(英) Chiba Shigeru

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貢献度任意

学籍番号推奨

題名

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(英) Notch activation induces the generation of functional NK cells from human cord blood CD34-positive cells devoid of IL-15.

副題

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要約

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(英) The development of NK cells from hematopoietic stem cells is thought to be dependent on IL-15. In this study, we demonstrate that stimulation of human cord blood CD34(+) cells by a Notch ligand, Delta4, along with IL-7, stem cell factor, and Fms-like tyrosine kinase 3 ligand, but no IL-15, in a stroma-free culture induced the generation of cells with characteristics of functional NK cells, including CD56 and CD161 Ag expression, IFN-gamma secretion, and cytotoxic activity against K562 and Jurkat cells. Addition of gamma-secretase inhibitor and anti-human Notch1 Ab to the culture medium almost completely blocked NK cell emergence. Addition of anti-human IL-15-neutralizing Ab did not affect NK cell development in these culture conditions. The presence of IL-15, however, augmented cytotoxicity and was required for a more mature NK cell phenotype. CD56(+) cells generated by culture with IL-15, but without Notch stimulation, were negative for CD7 and cytoplasmic CD3, whereas CD56(+) cells generated by culture with both Delta4 and IL-15 were CD7(+) and cytoplasmic CD3(+) from the beginning and therefore more similar to in vivo human NK cell progenitors. Together, these results suggest that Notch signaling is important for the physiologic development of NK cells at differentiation stages beyond those previously postulated.

キーワード

推奨

(英) Antigens, CD34
(英) Cell Differentiation
(英) Fetal Blood
(英) Flow Cytometry
(英) Fluorescent Antibody Technique
(英) Hematopoietic Stem Cells
(英) Humans
(英) Interleukin-15
(英) Killer Cells, Natural
(英) Lymphocyte Subsets
(英) Receptors, Notch
(英) Signal Transduction

発行所

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誌名

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The Journal of Immunology([The American Association of Immunologists])

(pISSN: 0022-1767, eISSN: 1550-6606)

ISSN	任意	1550-6606 ISSN: 0022-1767 (pISSN: 0022-1767, eISSN: 1550-6606) Title: Journal of immunology (Baltimore, Md. : 1950) Title(ISO): J Immunol Publisher: American Association of Immunologists (NLM Catalog) (Scopus) (CrossRef) (Scopus information is found. [need login])

巻

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182

号

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頁

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6168 6178

都市

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年月日

必須

2009年 5月 15日

URL

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DOI

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10.4049/jimmunol.0803036 (→Scopusで検索)

PMID

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19414770 (→Scopusで検索)

CRID

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WOS

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Scopus

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評価値

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被引用数

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指導教員

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備考

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(英) Article.Affiliation: Department of Cell Therapy and Transplantation Medicine, University of Tokyo Hospital, Tokyo, Japan.
(英) Article.PublicationTypeList.PublicationType: Journal Article
(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

徳島大学教育・研究者情報データベース(EDB)

Education and Research Database (EDB), Tokushima University