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著作: [岡村 裕彦]/[吉田 賀弥]/[落合 和彦]/[羽地 達次]/Reduction of protein phosphatase 2A Cα enhances bone formation and osteoblast differentiation through the expression of bone-specific transcription factor Osterix./[Bone]

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EID
233071
EOID
887446
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LastModified
2018年2月8日(木) 14:03:27
Operator
三木 ちひろ
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Owner
吉田 賀弥
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨 研究
共著種別 推奨
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著者 必須
  1. 岡村 裕彦
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  2. 吉田 賀弥([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健教育学]/[徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 落合 和彦
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 羽地 達次
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Reduction of protein phosphatase 2A Cα enhances bone formation and osteoblast differentiation through the expression of bone-specific transcription factor Osterix.

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(英) The serine/threonine protein phosphatase 2A (PP2A) participates in regulating many important physiological processes such as control of cell cycle, growth, and division. On the other hand, Osterix is a zinc-finger-containing transcription factor that is essential for the differentiation of osteoblasts and regulation of many bone-related genes. Here we examined the effect of okadaic acid (OA), a specific inhibitor of PP2A, on bone formation in vivo and the molecular mechanism regulated by PP2A Cα in osteoblast differentiation. Administration of 1nM OA to the calvarial region in mice increased bone mineral density, as shown by μCT, while histomorphological analysis showed an increase in mineral apposition and bone thickness in the same region. In addition, treatment with 1nM OA stimulated osteoblast differentiation and the expression of Osterix, bone sialoprotein (Bsp), and osteocalcin (OCN) in mouse osteoblastic MC3T3-E1 cells. Moreover, the expression and phosphatase activity of PP2A Cα was decreased in the initial step of osteoblast differentiation, which was in parallel with an increase in Osterix expression. To further clarify the role of PP2A Cα in osteoblast differentiation, we constructed PP2A knock-down cells by infecting MC3T3-E1 cells with a lentivirus expressing shRNA specific for the PP2A Cα. Accordingly, the silencing of PP2A Cα in MC3T3-E1 cells dramatically increased osteoblast differentiation and mineralization, which were accompanied with expressions of Osterix, Bsp, and OCN. Our data indicate that PP2A Cα plays an important role in the regulation of bone formation and osteoblast differentiation through the bone-related genes.

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誌名 必須 Bone(International Bone and Mineral Society)
(pISSN: 8756-3282, eISSN: 1873-2763)
ISSN 任意 1873-2763
ISSN: 8756-3282 (pISSN: 8756-3282, eISSN: 1873-2763)
Title: Bone
Title(ISO): Bone
Publisher: Elsevier BV
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 49
必須 3
必須 368 375
都市 任意
年月日 必須 2011年 6月 12日
URL 任意
DOI 任意 10.1016/j.bone.2011.06.004    (→Scopusで検索)
PMID 任意 21683816    (→Scopusで検索)
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WOS 任意 000293805100007
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  1. (英) Article.Affiliation: Department of Histology and Oral Histology, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima 770-8504, Japan.

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article