徳島大学 教育・研究者情報データベース(EDB)

1. (英) Kasai Yuka

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2. (英) Maegawa Masahiko

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3. (英) Yamamoto Satoshi

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4. (英) Kamada Masaharu

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5. 安井 敏之([徳島大学.大学院医歯薬学研究部.保健学域.保健科学部門.看護学系.生殖・更年期医療学]/[徳島大学.医学部.保健学科.看護学専攻.母性·小児看護学講座])

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6. 上村 浩一

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7. (英) Kobayashi Ayako

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8. (英) Kaneyama Masayo

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9. 谷 杏奈

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10. 松井 寿美佳

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11. 桑原 章

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12. 松崎 利也

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13. 古本 博孝

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14. 苛原 稔

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(英) Effects of raloxifene on the production of cytokines in stimulated whole blood in ex vivo and in vitro studies.

(英) The aims of this study were to determine the effects of raloxifene therapy on production of cytokines and in vitro effects of raloxifene on production of cytokines by whole blood cultures. We obtained samples of peripheral blood from 6 postmenopausal women with osteopenia at baseline and after 3 and 6 months of raloxifene therapy and 10 postmenopausal women who did not receive raloxifene therapy. Whole blood from raloxifene-treated women was stimulated with lipopolysaccharide (LPS) or phytohemeagglutinin (PHA). Whole blood from postmenopausal women who were not treated with raloxifene was preincubated with raloxifene at concentrations of 10(-10)-10(-7) M and then stimulated with LPS or PHA. Concentrations of IL-1β, IL-4, IL-6, IL-12p40, IL-12p70, TNF-α and IFN-γ in the supernatant were measured by respective ELISAs. In ex vivo cultures, raloxifene therapy inhibited LPS-stimulated production of IL-1β, IL-6, IL-12p40, IL-12p70 and TNF-α, but not PHA-stimulated production of IL-4 and IFN-γ. In in vitro cultures, raloxifene at a concentration (10(-9) M) inhibited LPS-stimulated production of IL-1β, IL-6 and IL-12p40 and PHA-stimulated production of IFN-γ. Raloxifene therapy decreases the production of IL-1β, IL-6, IL-12 and TNF-α but not that of IL-4 and IFN-γ, suggesting that modulation of cytokines could play a role in the mechanisms of the osteoprotective effect of raloxifene.

1. (英) Aged
2. (英) Bone Density Conservation Agents
3. (英) Bone Diseases, Metabolic
4. (英) Cell Survival
5. (英) Cytokines
6. (英) Female
7. (英) Humans
8. (英) Leukocytes, Mononuclear
9. (英) Menopause
10. (英) Middle Aged
11. (英) Raloxifene
12. (英) Selective Estrogen Receptor Modulators
13. (英) Time Factors

1. (英) Article.Affiliation: Department of Obstetrics and Gynecology, Tokushima Prefectural Central Hospital, Tokushima, Japan.

2. (英) Article.PublicationTypeList.PublicationType: In Vitro

3. (英) Article.PublicationTypeList.PublicationType: Journal Article

4. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't