徳島大学 教育・研究者情報データベース(EDB)

1. (英) Kumada Kazuki

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2. (英) Yao Ryoji

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3. (英) Kawaguchi Tokuichi

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4. (英) Karasawa Mika

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5. (英) Hoshikawa Yutaka

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6. (英) Ichikawa Koji

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7. (英) Sugitani Yoshinobu

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8. 井本 逸勢

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9. (英) Inazawa Johji

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10. (英) Sugawara Minoru

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11. (英) Yanagida Mitsuhiro

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12. (英) Noda Tetsuo

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(英) The selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase.

(英) Separase is an evolutionarily conserved protease that is essential for chromosome segregation and cleaves cohesin Scc1/Rad21, which joins the sister chromatids together. Although mammalian separase also functions in chromosome segregation, our understanding of this process in mammals is still incomplete. We generated separase knockout mice, reporting an essential function for mammalian separase. Separase-deficient mouse embryonic fibroblasts exhibited severely restrained increases in cell number, polyploid chromosomes, and amplified centrosomes. Chromosome spreads demonstrated that multiple chromosomes connected to a centromeric region. Live observation demonstrated that the chromosomes of separase-deficient cells condensed, but failed to segregate, although subsequent cytokinesis and chromosome decondensation proceeded normally. These results establish that mammalian separase is essential for the separation of centromeres, but not of the arm regions of chromosomes. Other cell cycle events, such as mitotic exit, DNA replication, and centrosome duplication appear to occur normally. We also demonstrated that heterozygous separase-deficient cells exhibited severely restrained increases in cell number with apparently normal mitosis in the absence of securin, which is an inhibitory partner of separase.

1. (英) Animals
2. (英) Carrier Proteins
3. (英) Cell Cycle Proteins
4. (英) Cell Proliferation
5. (英) Cells, Cultured
6. (英) Centromere
7. (英) Centrosome
8. (英) Chromosome Segregation
9. 細胞質分裂(cytokinesis)
10. (英) Endopeptidases
11. 女性(female)
12. (英) Fibroblasts
13. (英) Interphase
14. 男性(male)
15. (英) Mice
16. ノックアウトマウス(knockout mice)
17. (英) Mitosis
18. (英) Polyploidy

1. (英) Affiliation: Japanese Foundation for Cancer Research, Cancer Institute, Tokyo 135-8550, Japan.

2. (英) PublicationType: Journal Article

3. (英) PublicationType: Research Support, Non-U.S. Gov't