徳島大学 教育・研究者情報データベース(EDB)

1. (英) Kakuhata Rei

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2. (英) Watanabe Masahiro

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3. 山本 武範

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4. (英) Akamine Rie

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5. 山﨑 尚志([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.創薬科学系.衛生薬学]/[徳島大学.薬学部.創製薬科学科.創薬学講座.衛生薬学]/[徳島大学.薬学研究科.創薬科学専攻])

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6. 片岡 正俊

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7. (英) Fukuoka Satoshi

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8. 石川 満([独立行政法人 産業技術総合研究所])

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9. 大家 利彦(産業技術総合研究所健康工学研究センター)

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10. 馬場 嘉信

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11. (英) Hori Tomoshige

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12. 篠原 康雄([徳島大学.先端酵素学研究所.基幹研究部門])

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(英) Possible utilization of in vitro synthesized mRNAs specifically expressed in certain tissues as standards for quantitative evaluation of the results of microarray analysis.

(英) To examine the possible usefulness of in vitro synthesized RNA as standards in microarray analysis, we prepared full-length mRNAs encoded by 3 rat metabolic genes for heart/muscle type carnitine palmitoyltransferase I (M-CPTI), uncoupling protein (UCP1), and heart/muscle type fatty acid-binding protein (H-FABP). Artificial RNA samples were prepared by adding known amounts of these synthetic mRNAs to total RNA from rat liver, and transcript levels of various genes were compared between the prepared artificial RNA samples and total RNA samples of rat liver by using an Agilent oligo microarray system. Upon the addition of these synthetic RNAs, signals from the DNA spots corresponding to these 3 genes were elevated, but those from the DNA spots representing other genes were not markedly influenced. Using the ratio of the increase in signal intensity of DNA spot to the amount of added RNA, we estimated the expression levels of several genes and compared them with the absolute expression levels determined by calibrated Northern analysis. As a result, the absolute transcript levels of 3 genes encoding acidic ribosomal phosphoprotein P0, type-1 voltage-dependent anion channel (VDAC1), and type-2 glucose transporter (GLUT2) were successfully estimated by this procedure. Furthermore, genes specifically expressed in certain tissues such as UCP1 were concluded to be good candidates as standards for use in microarray analysis.

1. (英) Animals
2. (英) Base Sequence
3. (英) Carnitine O-Palmitoyltransferase
4. (英) DNA Primers
5. (英) DNA, Complementary
6. (英) Fatty Acid-Binding Proteins
7. (英) Gene Expression
8. (英) Ion Channels
9. (英) Mitochondrial Proteins
10. (英) Oligonucleotide Array Sequence Analysis
11. (英) RNA, Messenger
12. (英) Rats
13. (英) Tissue Distribution
14. (英) Transcription, Genetic

1. (英) Article.Affiliation: Institute for Genome Research, University of Tokushima, Kuramotocho-3, Tokushima 770-8503, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Evaluation Studies

3. (英) Article.PublicationTypeList.PublicationType: In Vitro

4. (英) Article.PublicationTypeList.PublicationType: Journal Article

5. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't