著作: Katsuda Chie/Niiyama Kanami/[尾華 絵里子]/[山本 武範]/Katou Yumiko/[片岡 正俊]/[大倉 一人]/[篠原 康雄]/Specific formation of trypsin-resistant micelles on a hydrophobic peptide observed with Triton X-100 but not with octylglucoside./[Biochimica et Biophysica Acta (BBA) - Biomembranes]
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削除する| 種別 | 必須 | 学術論文(審査論文) | |||
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| 言語 | 必須 | 英語 | |||
| 招待 | 推奨 | ||||
| 審査 | 推奨 | ||||
| カテゴリ | 推奨 | ||||
| 共著種別 | 推奨 | ||||
| 学究種別 | 推奨 | ||||
| 組織 | 推奨 | ||||
| 著者 | 必須 | ||||
| 題名 | 必須 |
(英) Specific formation of trypsin-resistant micelles on a hydrophobic peptide observed with Triton X-100 but not with octylglucoside. |
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| 副題 | 任意 | ||||
| 要約 | 任意 |
(英) The manner of interaction of the coat peptide of the Pf3 phage (Pf3 peptide) with lipid bilayers has been extensively studied. Presently, we designed a derivative of the Pf3 peptide, referred to as the DDRK peptide, and subjected it to trypsin digestion to understand its physicochemical properties. In the presence of Triton X-100 used for solubilization of the peptide, digestion of DDRK with trypsin caused specific cleavage at the lysine (Lys) residue in its N-terminal region but not at other Lys residues or at the arginine residue. As the N-terminal region of the DDRK peptide is relatively hydrophilic, but its remaining region is hydrophobic, this hydrophobic region of the peptide would be expected to be coated by Triton micelles. Thus, we propose that the presence of such micelles protected against cleavage there, leading to selective cleavage by trypsin of the DDRK peptide at its hydrophilic Lys residue in the N-terminal part of the molecule. However, such a protective effect on the DDRK peptide against trypsin digestion was not observed with octylglucoside. The observed results are important for better understanding of the manner of interaction between detergents and hydrophobic peptides. |
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| キーワード | 推奨 |
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| 発行所 | 推奨 | ||||
| 誌名 | 必須 |
Biochimica et Biophysica Acta (BBA) - Biomembranes([Elsevier Science])
(pISSN: 0005-2736, eISSN: 1879-2642)
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| 巻 | 必須 | 1798 | |||
| 号 | 必須 | 11 | |||
| 頁 | 必須 | 2090 2093 | |||
| 都市 | 任意 | ||||
| 年月日 | 必須 | 2010年 7月 18日 | |||
| URL | 任意 | ||||
| DOI | 任意 | 10.1016/j.bbamem.2010.07.013 (→Scopusで検索) | |||
| PMID | 任意 | 20646996 (→Scopusで検索) | |||
| CRID | 任意 | ||||
| Scopus | 任意 | ||||
| researchmap | 任意 | ||||
| 評価値 | 任意 | ||||
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| 指導教員 | 推奨 | ||||
| 備考 | 任意 |
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