著作: Purwanti, Nunuk/[辻 大輔]/Azlina, Ahmad/Karabasil, Ratko, Mileva/Javkhlan, Purevjav/[長谷川 敬展]/[姚 陳娟]/[赤松 徹也]/[伊藤 孝司]/[細井 和雄]/Induction of Sca-1 in the duct cells of the mouse submandibular gland by obstruction of the main excretory duct/[Journal of Oral Pathology & Medicine]
(英) Induction of Sca-1 in the duct cells of the mouse submandibular gland by obstruction of the main excretory duct
(英) The effect of ligation of the main excretory duct (MED) of the mouse submandibular gland (SMG) on the expression of Sca-1, a stem cell antigen, was examined by Western blotting and immunohistochemistry. By Western blotting, the expression of Sca-1 with a molecular weight of 18 kDa was identified in the normal gland. At 1 day post-ligation, the expression level of Sca-1 was strongly increased in the experimental gland and weakly in the contralateral gland, and such expression in both glands decreased at 6 days. By immunohistochemistry, Sca-1 was detected weakly in the apical membrane of excretory duct (ED) cells of the SMG under the normal condition. By duct ligation, Sca-1 became expressed strongly in most cells of the two major duct systems, i.e., the striated duct (SD) and granular convoluted tubules (GCT), but was not detected in the acinar (Ac) cells. By fluorescence-activated cell sorter (FACS) analysis, the number of side population (SP) cells in this gland was found to be increased by ligation. These results imply that Sca-1-positive cells may have a role in the duct cell proliferation in the regeneration step elicited by MED ligation-induced injury.
Journal of Oral Pathology & Medicine(International Association of Oral Pathologists/American Academy of Oral Pathology)
|年月日||必須||2011年 9月 初日|