著作: Fujisawa Koichi/Murakami Ryutaro/[堀口 大吾]/[野間 隆文]/Adenylate kinase isozyme 2 is essential for growth and development of Drosophila melanogaster./[Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology]
(英) Adenylate kinase isozyme 2 is essential for growth and development of Drosophila melanogaster.
(英) Adenylate kinases are phylogenetically widespread, highly conserved, and involved in energy metabolism and energy transfer. Of these, adenylate kinase (AK) isozyme 2 is uniquely localized in the mitochondrial intermembrane space and its physiological role remains largely unknown. In this study, we selected Drosophila melanogaster to analyze its role in vivo. AK isozyme cDNAs were cloned and their gene expressions were characterized in D. melanogaster. The deduced amino acid sequences contain highly conserved motifs for P-loop, NMP binding, and LID domains of AKs. In addition, the effects of AK2 gene knockout on phenotype of AK2 mutants were examined using P-element technology. Although homozygous AK2 mutated embryos developed without any visible defects, their growth ceased and they died before reaching the third instar larval stage. Maternally provided AK2 mRNA was detected in fertilized eggs, and weak AK2 activity was observed in first and second instar larvae of the homozygous AK2 mutants, suggesting that maternally provided AK2 is sufficient for embryonic development. Disappearance of AK2 activity during larval stages resulted in growth arrest and eventual death. These results demonstrate that AK2 plays a critical role in adenine nucleotide metabolism in the mitochondrial intermembrane space and is essential for growth in D. melanogaster.
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology([Elsevier])
|年月日||必須||2009年 1月 19日|