徳島大学 教育・研究者情報データベース(EDB)

1. 池原 敏孝([徳島文理大学])

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2. (英) Nishisako Hirotaka

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3. (英) Minami Yuki

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4. (英) Ichinose Sasaki Hiromi

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5. (英) Shiraishi Tairo

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6. 北村 光夫([徳島大学.技術支援部])

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7. 庄野 正行([徳島大学.大学院医歯薬学研究部.医学域.総合研究支援センター])

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8. 芳地 一

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9. 川添 和義

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10. 水口 和生

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11. 吉﨑 和男

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12. 木内 陽介

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13. 宮本 博司([徳島文理大学])

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(英) Effects of exposure to a time-varying 1.5T magnetic field on the neurotransmitter-activated increase in intracellular Ca(2+) in relation to actin fiber and mitochondrial functions in bovine adrenal chromaffin cells.

(英) BACKGROUND: It has been reported that exposure to electromagnetic fields influences intracellular signal transduction. We studied the effects of exposure to a time-varying 1.5T magnetic field on membrane properties, membrane cation transport and intracellular Ca(2+) mobilization in relation to signals. We also studied the mechanism of the effect of exposure to the magnetic field on intracellular Ca(2+) release from Ca(2+) stores in adrenal chromaffin cells. METHODS: We measured the physiological functions of ER, actin protein, and mitochondria with respect to a neurotransmitter-induced increase in Ca(2+) in chromaffin cells exposed to the time-varying 1.5T magnetic field for 2h. RESULTS: Exposure to the magnetic field significantly reduced the increase in [Ca(2+)]i. The exposure depolarized the mitochondria membrane and lowered oxygen uptake, but did not reduce the intracellular ATP content. Magnetic field-exposure caused a morphological change in intracellular F-actin. F-actin in exposed cells seemed to be less dense than in control cells, but the decrease was smaller than that in cytochalasin D-treated cells. The increase in G-actin (i.e., the decrease in F-actin) due to exposure was recovered by jasplakinolide, but inhibition of Ca(2+) release by the exposure was unaffected. CONCLUSIONS AND GENERAL SIGNIFICANCE: These results suggest that the magnetic field-exposure influenced both the ER and mitochondria, but the inhibition of Ca(2+) release from ER was not due to mitochondria inhibition. The effect of eddy currents induced in the culture medium may indirectly influence intracellular actin and suppress the transient increase in [Ca(2+)]i.

1. (英) Article.Affiliation: Department of Human Welfare, Faculty of Health and Welfare, Tokushima Bunri University, Yamashiro-cho, Tokushima 770-8514, Japan; Department of Physiology, Institute of Health BioSciences, The University of Tokushima Graduate School, Tokushima 770-8503, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article