徳島大学 教育・研究者情報データベース(EDB)

Education and Research Database (EDB), Tokushima University

徳島大学ウェブサイトへのリンク

著作: [銭 志栄]/[佐野 壽昭]/[吉本 勝彦]/[山田 正三]/Ishizuka Akira/[水澤 典子]/[堀口 英久]/[廣川 満良]/Asa Sylvia L/Inactivation of RASSF1A tumor suppressor gene by aberrant promoter hypermethylation in human pituitary adenomas./[Laboratory Investigation; a Journal of Technical Methods and Pathology]

ヘルプを読む

「著作」(著作(著書,論文,レター,国際会議など))は,研究業績にかかる著作(著書,論文,レター,国際会議など)を登録するテーブルです. (この情報が属するテーブルの詳細な定義を見る)

  • 項目名の部分にマウスカーソルを置いて少し待つと,項目の簡単な説明がツールチップ表示されます.

この情報をEDB閲覧画面で開く

EID
212964
EOID
654433
Map
0
LastModified
2012年8月28日(火) 22:21:22
Operator
大家 隆弘
Avail
TRUE
Censor
0
Owner
吉本 勝彦
Read
継承
Write
継承
Delete
継承
種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨
共著種別 推奨
学究種別 推奨 博士後期課程学生による研究報告
組織 推奨
著者 必須
  1. 銭 志栄
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 佐野 壽昭
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 吉本 勝彦
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 山田 正三
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Ishizuka Akira
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. 水澤 典子([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔生命科学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. 堀口 英久
    役割 任意
    貢献度 任意
    学籍番号 推奨
  8. 廣川 満良
    役割 任意
    貢献度 任意
    学籍番号 推奨
  9. (英) Asa Sylvia L
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Inactivation of RASSF1A tumor suppressor gene by aberrant promoter hypermethylation in human pituitary adenomas.

副題 任意
要約 任意

(英) Aberrant promoter methylation and resultant silencing of several tumor suppressor genes play an important role in the pathogenesis of many tumor types. The human Ras association domain family 1A gene (RASSF1A), recently cloned from the lung tumor locus at 3p21.3, was shown to be frequently inactivated by hypermethylation of its promoter region in a number of malignancies. We have investigated the expression and epigenetic changes of this novel universal tumor suppressor gene in pituitary adenomas and correlated the data with clinicopathologic findings. Fresh frozen normal pituitary tissues and 52 primary pituitary adenomas including all major types were examined. Methylation-specific polymerase chain reaction (MSP), combined bisulfite restriction analysis (COBRA), bisulfite sequencing and semiquantitative reverse transcription-polymerase chain reaction were used to analyze DNA promoter methylation status and the mRNA expression of RASSF1A, respectively. High levels of RASSF1A transcript and no methylation of the RASSF1A promoter were found in normal pituitary tissues. RASSF1A promoter methylation was detected in 20 of 52 (38%) adenomas including all major types of pituitary adenomas. However, a lower frequency of methylation of the RASSF1A promoter was found in gonadotroph cell adenomas (15%) compared with growth hormone cell, prolactin cell, or adrenocorticotropic hormone cell adenomas (54, 46 and 50%, respectively). Methylation frequency was higher in the most aggressive adenomas (87% in grade IV tumors, P=0.0163). In addition, methylation of the RASSF1A promoter potentially correlated with higher labeling index of the proliferation marker Ki-67 (P=0.1475). Loss or significant reduction of RASSF1A messenger RNA transcripts was identified in 18 of 20 (90%) adenomas with hypermethylation of RASSF1A (P<0.0001). Our data suggest that promoter hypermethylation of RASSF1A and resultant alterations of RASSF1A expression may play a critical role in pituitary tumorigenesis and may be involved in tumor progression.

キーワード 推奨
  1. (英) Adenoma
  2. (英) Base Sequence
  3. (英) DNA Methylation
  4. (英) Female
  5. (英) Humans
  6. 免疫組織化学(immunohistochemistry)
  7. (英) Male
  8. (英) Molecular Sequence Data
  9. (英) Pituitary Neoplasms
  10. (英) Promoter Regions, Genetic
  11. (英) Reverse Transcriptase Polymerase Chain Reaction
  12. (英) Tumor Suppressor Proteins
発行所 推奨
誌名 必須 Laboratory Investigation; a Journal of Technical Methods and Pathology(United States and Canadian Academy of Pathology)
(pISSN: 0023-6837, eISSN: 1530-0307)
ISSN 任意 0023-6837
ISSN: 0023-6837 (pISSN: 0023-6837, eISSN: 1530-0307)
Title: Laboratory investigation; a journal of technical methods and pathology
Title(ISO): Lab Invest
Publisher: Springer Science+Business Media
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 85
必須 4
必須 464 473
都市 任意
年月日 必須 2005年 4月 15日
URL 任意
DOI 任意 10.1038/labinvest.3700248    (→Scopusで検索)
PMID 任意 15711568    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Affiliation: Department of Pathology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan. zrqian@basic.med.tokushima-u.ac.jp

  2. (英) PublicationType: Journal Article