徳島大学 教育・研究者情報データベース(EDB)

1. (英) Erdenebayar Namjil

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2. 前川 洋一

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3. 西田 純

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4. 北村 明子

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5. 安友 康二([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.病理系.生体防御医学])

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(英) Protein-tyrosine phosphatase-kappa regulates CD4+ T cell development through ERK1/2-mediated signaling.

(英) T cells express diverse antigen-specific receptors and are required for eradicating pathogens and transformed cells. T cells expressing CD4 acquire helper effector functions and those expressing CD8 exert cytotoxic activity after antigen recognition. The protein-tyrosine phosphatase, receptor type kappa (PTPRKappa) is mutated in LEC rats, resulting in impaired CD4(+) T cell development in the thymus. However, the molecular mechanism of PTPRK controlling CD4(+) T cell development remains unclear. We demonstrate herein that inhibition of PTPRK by transducing a dominant negative form of the intracellular domain of PTPRK (PTPRK-ICD-DN) in bone marrow-derived stem cells suppresses the development of CD4(+) T cells. The inhibition of PTPRK by PTPRK-ICD-DN or short-hairpin RNA for PTPRK attenuates ERK1/2 phosphorylation in T cells after PMA and ionomycin stimulation. Total thymocytes from LEC rats also showed weaker phosphorylation of ERK1/2 after PMA and ionomycin stimulation than control thymocytes. Furthermore, inhibition of PTPRK by PTPRK-ICD-DN suppressed MEK1/2 and c-Raf phosphorylation, which is required for ERK1/2 phosphorylation. These data indicate that PPTRK positively regulates ERK1/2 phosphorylation, which impacts CD4(+) T cell development.

1. (英) Animals
2. (英) CD4-Positive T-Lymphocytes
3. (英) Humans
4. (英) Jurkat Cells
5. (英) MAP Kinase Kinase 1
6. (英) MAP Kinase Kinase 2
7. (英) Mice
8. (英) Mitogen-Activated Protein Kinase 1
9. (英) Mitogen-Activated Protein Kinase 3
10. リン酸化(phosphorylation)
11. (英) Rats
12. (英) Receptor-Like Protein Tyrosine Phosphatases, Class 2
13. シグナル伝達(signal transduction)

1. (英) Article.Affiliation: Department of Immunology and Parasitology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't