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著作: Ushizawa Koichi/Herath B. Chandana/Kaneyama Kanako/Shiojima Satoshi/Hirasawa Akira/Takahashi Toru/Imai Kei/[落合 和彦]/Tokunaga Tomoyuki/Tsunoda Yukio/Tsujimoto Gozoh/Hashizume Kazuyoshi/cDNA microarray analysis of bovine embryo gene expression profiles during the pre-implantation period./[Reproductive Biology and Endocrinology : RB&E]

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EID
210017
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654235
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2012年8月28日(火) 21:39:29
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大家 隆弘
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[副研究部長]/[徳島大学.大学院ヘルスバイオサイエンス研究部]
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Ushizawa Koichi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Herath B. Chandana
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Kaneyama Kanako
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Shiojima Satoshi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Hirasawa Akira
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Takahashi Toru
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. (英) Imai Kei
    役割 任意
    貢献度 任意
    学籍番号 推奨
  8. 落合 和彦
    役割 任意
    貢献度 任意
    学籍番号 推奨
  9. (英) Tokunaga Tomoyuki
    役割 任意
    貢献度 任意
    学籍番号 推奨
  10. (英) Tsunoda Yukio
    役割 任意
    貢献度 任意
    学籍番号 推奨
  11. (英) Tsujimoto Gozoh
    役割 任意
    貢献度 任意
    学籍番号 推奨
  12. (英) Hashizume Kazuyoshi
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) cDNA microarray analysis of bovine embryo gene expression profiles during the pre-implantation period.

副題 任意
要約 任意

(英) BACKGROUND: After fertilization, embryo development involves differentiation, as well as development of the fetal body and extra-embryonic tissues until the moment of implantation. During this period various cellular and molecular changes take place with a genetic origin, e.g. the elongation of embryonic tissues, cell-cell contact between the mother and the embryo and placentation. To identify genetic profiles and search for new candidate molecules involved during this period, embryonic gene expression was analyzed with a custom designed utero-placental complementary DNA (cDNA) microarray. METHODS: Bovine embryos on days 7, 14 and 21, extra-embryonic membranes on day 28 and fetuses on days 28 were collected to represent early embryo, elongating embryo, pre-implantation embryo, post-implantation extra-embryonic membrane and fetus, respectively. Gene expression at these different time points was analyzed using our cDNA microarray. Two clustering algorithms such as k-means and hierarchical clustering methods identified the expression patterns of differentially expressed genes across pre-implantation period. Novel candidate genes were confirmed by real-time RT-PCR. RESULTS: In total, 1,773 individual genes were analyzed by complete k-means clustering. Comparison of day 7 and day 14 revealed most genes increased during this period, and a small number of genes exhibiting altered expression decreased as gestation progressed. Clustering analysis demonstrated that trophoblast-cell-specific molecules such as placental lactogens (PLs), prolactin-related proteins (PRPs), interferon-tau, and adhesion molecules apparently all play pivotal roles in the preparation needed for implantation, since their expression was remarkably enhanced during the pre-implantation period. The hierarchical clustering analysis and RT-PCR data revealed new functional roles for certain known genes (dickkopf-1, NPM, etc) as well as novel candidate genes (AW464053, AW465434, AW462349, AW485575) related to already established trophoblast-specific genes such as PLs and PRPs. CONCLUSIONS: A large number of genes in extra-embryonic membrane increased up to implantation and these profiles provide information fundamental to an understanding of extra-embryonic membrane differentiation and development. Genes in significant expression suggest novel molecules in trophoblast differentiation.

キーワード 推奨
  1. (英) Animals
  2. (英) Cattle
  3. (英) Cluster Analysis
  4. (英) DNA, Complementary
  5. (英) Embryo, Mammalian
  6. (英) Embryonic Development
  7. (英) Female
  8. (英) Fetus
  9. (英) Gene Expression Profiling
  10. (英) Gene Expression Regulation, Developmental
  11. (英) Genes
  12. (英) Gestational Age
  13. (英) Membranes
  14. (英) Microarray Analysis
  15. (英) Nucleic Acid Amplification Techniques
発行所 推奨
誌名 必須 Reproductive Biology and Endocrinology : RB&E([BioMed Central Ltd.])
(eISSN: 1477-7827)
ISSN 任意 1477-7827
ISSN: 1477-7827 (eISSN: 1477-7827)
Title: Reproductive biology and endocrinology : RB&E
Title(ISO): Reprod Biol Endocrinol
Publisher: BioMed Central
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 2
必須
必須 77 77
都市 任意
年月日 必須 2004年 11月 24日
URL 任意
DOI 任意 10.1186/1477-7827-2-77    (→Scopusで検索)
PMID 任意 15560851    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Reproductive Biology and Technology Laboratory, Developmental Biology Department, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba, Ibaraki 305-8602, Japan. ushizawa@affrc.go.jp <ushizawa@affrc.go.jp>

  2. (英) Article.PublicationTypeList.PublicationType: Comparative Study

  3. (英) Article.PublicationTypeList.PublicationType: Journal Article

  4. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  5. (英) OtherID: PMC535809