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著作: Yoshikawa Yasunaga/Morimatsu Masami/[落合 和彦]/Nagano Masashi/Tomioka Yukiko/Sasaki Nobuo/Hashizume Kazuyoshi/Iwanaga Toshihiko/Novel variations and loss of heterozygosity of BRCA2 identified in a dog with mammary tumors./[American Journal of Veterinary Research]

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EID
210008
EOID
654230
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0
LastModified
2012年8月28日(火) 21:38:37
Operator
大家 隆弘
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[副研究部長]/[徳島大学.大学院ヘルスバイオサイエンス研究部]
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Yoshikawa Yasunaga
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Morimatsu Masami
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 落合 和彦
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Nagano Masashi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Tomioka Yukiko
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Sasaki Nobuo
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. (英) Hashizume Kazuyoshi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  8. (英) Iwanaga Toshihiko
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Novel variations and loss of heterozygosity of BRCA2 identified in a dog with mammary tumors.

副題 任意
要約 任意

(英) OBJECTIVE: To establish novel polymorphic markers for analysis of loss of heterozygosity (LOH), so as to study the possible involvement of BRCA2 in mammary tumors obtained from dogs. SAMPLE POPULATION: Blood samples, mammary gland specimens, or mammary tumors from 3 tumor-bearing dogs and 10 tumor-free dogs. PROCEDURES: Nucleotide sequence analysis was performed with a DNA autosequencer. Loss of heterozygosity analysis was performed for markers established in the present study. The expression level of canine BRCA2 was quantified by real-time PCR analysis. RESULTS: 3 novel microsatellite markers with high heterozygosity rates (> 50%) were established, and the previously reported marker for canine BRCA2 gene locus was improved. These markers were used for the analysis of DNA from formalin-fixed and paraffin-embedded samples. By use of these markers, LOH in canine BRCA2 was identified as a result of recombination. In mammary tumor DNA that corresponded to the LOH-positive dog, the level of canine BRCA2 expression was decreased compared with that of nonneoplastic mammary gland tissue; the open reading frame contained 4 missense variations, 1 insertion variation, and 1 silent variation, some of which were localized to functional domains. CONCLUSIONS AND CLINICAL RELEVANCE: 3 novel polymorphic markers were developed for LOH analysis of canine BRCA2 and identified a dog with LOH with some variations in the functional domains. These markers could be useful for assessing the relevance of BRCA2 variation in mammary tumors of dogs.

キーワード 推奨
  1. (英) Animals
  2. (英) BRCA2 Protein
  3. (英) Base Sequence
  4. (英) Cloning, Molecular
  5. (英) DNA Primers
  6. (英) DNA, Complementary
  7. (英) DNA, Neoplasm
  8. (英) Dog Diseases
  9. (英) Dogs
  10. (英) Female
  11. (英) Genetic Variation
  12. (英) Loss of Heterozygosity
  13. (英) Mammary Neoplasms, Animal
  14. (英) Open Reading Frames
発行所 推奨
誌名 必須 American Journal of Veterinary Research(American Veterinary Medical Association)
(pISSN: 0002-9645, eISSN: 1943-5681)
ISSN 任意 0002-9645
ISSN: 0002-9645 (pISSN: 0002-9645, eISSN: 1943-5681)
Title: American journal of veterinary research
Title(ISO): Am J Vet Res
Publisher: American Veterinary Medical Association
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 69
必須 10
必須 1323 1328
都市 任意
年月日 必須 2008年 10月 初日
URL 任意
DOI 任意 10.2460/ajvr.69.10.1323    (→Scopusで検索)
PMID 任意 18828690    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Laboratory of Cytology and Histology, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan.

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't