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著作: Suzuki Yoshihiro/[吉丸 哲郎]/Matsui Takashi/Inoue Toshio/Niide Osamu/Nunomura Satoshi/Ra Chisei/Fc epsilon RI signaling of mast cells activates intracellular production of hydrogen peroxide: role in the regulation of calcium signals./[The Journal of Immunology]

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EID
207377
EOID
698809
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0
LastModified
2013年8月3日(土) 19:21:33
Operator
大家 隆弘
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Owner
吉丸 哲郎
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Suzuki Yoshihiro
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 吉丸 哲郎([徳島大学.先端酵素学研究所.重点研究部門])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Matsui Takashi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Inoue Toshio
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Niide Osamu
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Nunomura Satoshi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. (英) Ra Chisei
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Fc epsilon RI signaling of mast cells activates intracellular production of hydrogen peroxide: role in the regulation of calcium signals.

副題 任意
要約 任意

(英) Earlier studies, including our own, revealed that activation of mast cells is accompanied by production of reactive oxygen species (ROS) that help to mediate the release of the inflammatory mediators, including histamine and eicosanoids. However, little is known about the mechanisms of ROS production, including the species of oxidants produced. In this study we show that in both the RBL-2H3 mast cell line and bone marrow-derived mast cells, FcepsilonRI cross-linking stimulates intracellular oxidative burst, including hydrogen peroxide (H(2)O(2)) production, as defined with the oxidant-sensitive dyes dichlorofluorescein and scopoletin and the selective scavenger ebselen (2-phenyl-1,2-benzisoselenazol-3(2H)-one). The oxidative burst was observed immediately after stimulation and was most likely due to an NAD(P)H oxidase. Experiments using selective pharmacological inhibitors demonstrated that activation of tyrosine kinases and phosphatidylinositol-3-kinase is required for induction of the oxidative burst. Blockade of the oxidative burst by diphenyleneiodonium impaired the release of preformed granular mediators, such as histamine and beta-hexosaminidase, and the secretion of newly synthesized leukotriene C(4), whereas selective scavenging H(2)O(2) by ebselen impaired leukotriene C(4) secretion, but not degranulation. Sustained elevation of cytosolic calcium through store-operated calcium entry was totally abolished when ROS production was blocked. In contrast, selective depletion of H(2)O(2) caused a considerable decrease and delay of the calcium response. Finally, tyrosine phosphorylation of phospholipase Cgamma and the linker for activation of T cells, an event required for calcium influx, was suppressed by diphenyleneiodonium and ebselen. These studies demonstrate that activation of the intracellular oxidative burst is an important regulatory mechanism of mast cell responses.

キーワード 推奨
  1. (英) 1-Phosphatidylinositol 3-Kinase
  2. (英) Adaptor Proteins, Signal Transducing
  3. (英) Animals
  4. (英) Antioxidants
  5. (英) Calcium
  6. (英) Calcium Signaling
  7. (英) Carrier Proteins
  8. (英) Cell Line, Tumor
  9. (英) Hydrogen Peroxide
  10. (英) Intracellular Fluid
  11. (英) Mast Cells
  12. (英) Membrane Proteins
  13. (英) Mice
  14. (英) NADH, NADPH Oxidoreductases
  15. (英) NADPH Oxidase
  16. (英) Onium Compounds
  17. (英) Phospholipase C gamma
  18. (英) Phosphoproteins
  19. (英) Phosphorylation
  20. (英) Phosphotyrosine
  21. (英) Protein-Tyrosine Kinases
  22. (英) Rats
  23. (英) Reactive Oxygen Species
  24. (英) Receptors, IgE
  25. (英) Respiratory Burst
  26. (英) T-Lymphocytes
  27. (英) Type C Phospholipases
発行所 推奨
誌名 必須 The Journal of Immunology([The American Association of Immunologists])
(pISSN: 0022-1767, eISSN: 1550-6606)
ISSN 任意 0022-1767
ISSN: 0022-1767 (pISSN: 0022-1767, eISSN: 1550-6606)
Title: Journal of immunology (Baltimore, Md. : 1950)
Title(ISO): J Immunol
Publisher: American Association of Immunologists
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 171
必須 11
必須 6119 6127
都市 任意
年月日 必須 2003年 12月 1日
URL 任意
DOI 任意
PMID 任意 14634127    (→Scopusで検索)
NAID 任意
WOS 任意 000186767200060
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Affiliation: Division of Molecular Cell Immunology and Allergology, Nihon University Graduate School of Medical Sciences, Tokyo, Japan. ysuzuki@med.nihon-u.ac.jp

  2. (英) PublicationType: Journal Article

  3. (英) PublicationType: Research Support, Non-U.S. Gov't