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著作: Hidaka Kyoko/[新田 剛]/Sugawa Ryo/Schwartz Robert J/Amagai Takashi/Nitta Sachiko/[高浜 洋介]/Morisaki Takayuki/Differentiation of Pharyngeal Endoderm and Derivatives from Mouse Embryonic Stem Cells./[Stem Cells and Development]

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EID
206730
EOID
758436
Map
0
LastModified
2014年11月28日(金) 11:25:30
Operator
松井 栄里
Avail
TRUE
Censor
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Owner
高浜 洋介
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨 研究
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Hidaka Kyoko
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 新田 剛
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Sugawa Ryo
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Schwartz Robert J
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Amagai Takashi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Nitta Sachiko
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. 高浜 洋介
    役割 任意
    貢献度 任意
    学籍番号 推奨
  8. (英) Morisaki Takayuki
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Differentiation of Pharyngeal Endoderm and Derivatives from Mouse Embryonic Stem Cells.

副題 任意
要約 任意

(英) Embryonic stem cells are considered to be a good in vitro tool to study the induction of various cell types including cardiomyocytes; however, induction of the pharyngeal endoderm (PE), the underlying heart-forming region, in vivo has been scarcely reported. In the present study, we found that many PE-related genes, such as Paxl, Pax9, Sixl, and Tbxl, were up-regulated in cardiomyocyte-rich embryoid bodies (EBs). The third pouch-related genes including Hoxa3, Foxn1, and Aire, which are crucial for thymus development and function, were also detected in later stages. Nkx2.5, a cardiac transcription factor gene, is known to be transiently expressed in the PE. By crossing Nkx2.5-Cre mice with Cre-dependent EGFP reporter mice, we found that Nkx2.5(+) lineage exclusively contributed to thymic epithelial cell development, followed by thymus development. Gene expression analysis using Nkx2.5-EGFP ES cells also revealed that PE-related mRNAs were specifically enriched in the transiently appearing E-cadherin(+)Nkx2.5(+) cell fraction. Interestingly, the EB-derived cells were found capable of supporting T-cell differentiation to CD4 or CD8 double-positive cells in a reaggregation organ culture in vitro. Our results suggest that EBs contain cells that resemble third pharyngeal pouch endoderm and confer a thymus-like microenvironment.

キーワード 推奨
  1. (英) Animals
  2. (英) Cell Differentiation
  3. (英) Cell Lineage
  4. (英) Cells, Cultured
  5. (英) Coculture Techniques
  6. (英) Embryoid Bodies
  7. (英) Embryonic Stem Cells
  8. (英) Endoderm
  9. (英) Homeodomain Proteins
  10. (英) Kidney
  11. (英) Mice
  12. (英) Mice, Transgenic
  13. (英) Pharynx
  14. (英) Thymus Gland
  15. (英) Transcription Factors
発行所 推奨
誌名 必須 Stem Cells and Development(Mary Ann Liebert, Inc.)
(pISSN: 1547-3287, eISSN: 1557-8534)
ISSN 任意 1557-8534
ISSN: 1547-3287 (pISSN: 1547-3287, eISSN: 1557-8534)
Title: Stem cells and development
Title(ISO): Stem Cells Dev
Publisher: Mary Ann Liebert Inc.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 19
必須 11
必須 1735 1743
都市 任意
年月日 必須 2010年 11月 末日
URL 任意
DOI 任意 10.1089/scd.2009.0466    (→Scopusで検索)
PMID 任意 20230268    (→Scopusで検索)
NAID 任意
WOS 任意 000283544200009
Scopus 任意 2-s2.0-78049383117
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Bioscience, National Cardiovascular Center Research Institute, Suita, Osaka, Japan.

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't