著作: Kamiya Takashi/[谷本 幸太郎]/Tanne Yuki/Lin YY/Kunimatsu Ryo/Yoshioka Motoko/Tanaka Nobuaki/[田中 栄二]/[丹根 一夫]/Effects of mechanical stimuli on the synthesis of superficial zone protein in chondrocytes/[Journal of Biomedical Materials Research. Part A]
(英) Effects of mechanical stimuli on the synthesis of superficial zone protein in chondrocytes
(英) Superficial zone protein (SZP) has been demonstrated to contribute to the boundary lubrication in synovial joints. This study was designed to clarify the modulation of SZP expression by mechanical stress in articular chondrocytes. Cyclic tensile strains of 7 and 21% cell elongation were applied to cultured chondrocytes obtained from porcine mandibular condylar cartilage. The mRNA levels of SZP, IL-1 beta, and TGF-beta1 were examined by a quantitative real-time PCR analysis. Protein level of SZP was examined by Western blotting. The SZP mRNA level was significantly upregulated after 12, 24, and 48 h by 7% elongation. Although SZP mRNA level was upregulated by 21% elongation after 12 h, it decreased to a lower level than the control after 48 h. The TGF-beta1 mRNA level exhibited an almost similar change to SZP. The IL-1 beta mRNA level was not changed markedly by 7% elongation. However, the IL-1 beta mRNA level was significantly increased by a 12-h application of 21% elongation. Western blot analysis revealed that the SZP expression was increased by 7% elongation, but decreased remarkably by 21% elongation. It is suggested from these findings that the SZP expression level in the chondrocytes is enhanced by optimal mechanical stimuli, but inhibited by excessive loading partly affected by TGF-beta1 and IL-1 beta, leading to the deterioration of joint lubrication. (c) 2009 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research. Part A([John Wiley & Sons, Inc.])
|年月日||必須||2010年 2月 末日|