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著作: Komuro Ryutaro/[佐々木 卓也]/Takaishi Kenji/[織田 聡]/Takai Yoshimi/Involvement of Rho and Rac small G proteins and Rho GDI in Ca2+-dependent exocytosis from PC12 cells./[Genes to Cells]

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EID
204240
EOID
840186
Map
0
LastModified
2016年11月22日(火) 13:46:53
Operator
三木 ちひろ
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TRUE
Censor
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Owner
佐々木 卓也
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Komuro Ryutaro
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 佐々木 卓也([徳島大学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Takaishi Kenji
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 織田 聡
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Takai Yoshimi
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Involvement of Rho and Rac small G proteins and Rho GDI in Ca2+-dependent exocytosis from PC12 cells.

副題 任意
要約 任意

(英) The Rho small G protein family, which includes the Rho, Rac and Cdc42 subfamilies, is implicated in various cell functions such as cell shape change, cell motility and cytokinesis, through the reorganization of actin filaments. Rho GDI is an inhibitory regulator of the Rho small G protein family and inhibits the Rho family dependent cell functions. Reorganization of actin filaments is also known to regulate Ca2+-dependent exocytosis. We have examined here whether the Rho family members are also involved in Ca2+-dependent exocytosis. We have found, by the use of the human growth hormone (GH) co-expression assay system on PC12 cells, that overexpression of Rho GDI inhibits high K+-induced, Ca2+-dependent GH release. This inhibitory action of Rho GDI is restored by co-expression of a dominant active mutant of RhoA or Rac1, but not of a dominant active mutant of Cdc42. C3 transferase, known to ADP-ribosylate Rho and to inhibit its function, also inhibits this GH release. Overexpression of a dominant active mutant of RhoA or Rac1 alone shows only a small effect on GH release. Moreover, immunocytochemical studies show that the overexpression of Rho GDI prevents a partial disruption of the cortical actin network which accompanies exocytosis. These results suggest that RhoA, Rac1 and Rho GDI are involved in Ca2+-dependent exocytosis at least partly through the reorganization of actin filaments, and that the activation of RhoA or Rac1 alone is not sufficient for this reaction.

キーワード 推奨
  1. (英) ADP Ribose Transferases
  2. (英) Actin Cytoskeleton
  3. (英) Actins
  4. (英) Animals
  5. (英) Botulinum Toxins
  6. カルシウム(calcium)
  7. (英) Cell Cycle Proteins
  8. (英) Exocytosis
  9. (英) GTP-Binding Proteins
  10. (英) Growth Hormone
  11. (英) Guanine Nucleotide Dissociation Inhibitors
  12. (英) Mutation
  13. (英) PC12 Cells
  14. カリウム(potassium)
  15. (英) Rats
  16. (英) Recombinant Proteins
  17. (英) cdc42 GTP-Binding Protein
  18. (英) rac GTP-Binding Proteins
  19. (英) rho GTP-Binding Proteins
  20. (英) rhoA GTP-Binding Protein
発行所 推奨
誌名 必須 Genes to Cells([日本分子生物学会])
(pISSN: 1356-9597, eISSN: 1365-2443)
ISSN 任意 1356-9597
ISSN: 1356-9597 (pISSN: 1356-9597, eISSN: 1365-2443)
Title: Genes to cells : devoted to molecular & cellular mechanisms
Title(ISO): Genes Cells
Supplier: Molecular Biology Society of Japan
Publisher: Wiley Publishing
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 1
必須 10
必須 943 951
都市 任意
年月日 必須 1996年 10月 初日
URL 任意
DOI 任意 10.1046/j.1365-2443.1996.760276.x    (→Scopusで検索)
PMID 任意 9077452    (→Scopusで検索)
NAID 任意
WOS 任意 A1996WC16200006
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita, Japan.

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't