徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.工学部.生物工学科.生物機能工学講座

1. 金品 昌志

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2. (英) Miyata Takahiro / (日) 宮田 隆弘 / (読) みやた たかひろ

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3. 松木 均([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])

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4. 佐竹 弘

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5. (英) Kuroki Motohiko / (日) 黒木 元彦 / (読) くろき もとひこ

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(英) Solubilization Study of Local Anesthetics into Sodium Dodecyl Sulfate Micelle Using Anesthetic Cation Selective Electrodes

(英) The free concentrations of local anesthetic cations in equilibrium with sodium dodecyl sulfate (SDS) micelle which solubilized the anesthetic were determined by using ion-selective electrodes sensitive to local anesthetics, procaine (PC), lidocaine (LC), and mepivacaine (MC). Solubilizate distribution between water and SDS micelle was analyzed by means of the stepwise mass-action model. Association constant, K(1), was found to depend upon the anesthetic concentration, which decreased exponentially as the concentration of free anesthetic increased. Therefore, K(1) should include the interaction function &phi;(A) as K(1)=K(int)exp{-&phi;(A)} where K(int) is an intrinsic association constant. &phi;(A) is an increasing function of the anesthetic concentration, which means that occupation of a solubilization site by a local anesthetic cation makes sequential solubilization more difficult. The binding affinity of an anesthetic with SDS micelle increased in the following order PC<LC<MC.The critical micelle concentration (CMC) of mixed micelle was determined as a function of the concentration of free anesthetic. The CMC decreased with an increasing amount of anesthetics solubilized. All the anesthetic compositions in the micelle calculated thermodynamically from the CMC data were larger than the corresponding ones in the aqueous phase. Although the local anesthetics used here do not form micelles by themselves, the CMC vs composition curve can be regarded as a part of a micellar phase diagram showing the negative azeotropic behavior, which reflects the attractive interaction between the anionic surfactant micelle and the local anesthetic cation.

(日) ドデシル硫酸ナトリウムミセルに可溶化した3種類の局所麻酔薬(プロカイン，リドカイン，メピバカイン)と水溶液中に平衡に存在するそれぞれの遊離局所麻酔薬カチオン濃度を局所麻酔薬選択性電極を用いて決定した．水中とミセル中の可溶化物質の分布を段階的質量作用モデルを用いて解析した．ミセルとの結合親和性はプロカイン<リドカイン<メピバカインの順序で増大した．さらに臨界ミセル濃度の組成依存性からミセル中の麻酔薬組成を求めた．ミセル形成の相図は負の共沸型となり，陰イオン性界面活性剤ミセルと陽イオン性局所麻酔薬カチオン間の強い引力的相互作用を示した．

1. (英) Article.PublicationTypeList.PublicationType: Journal Article