徳島大学 教育・研究者情報データベース(EDB)

1. (英) Matsunaga Takuya

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2. (英) Tanaka Ikuta

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3. (英) Kobune Masayoshi

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4. (英) Kawano Yutaka

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5. (英) Tanaka Maki

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6. (英) Kuribayashi Kageaki

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7. (英) Iyama Satoshi

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8. (英) Sato Tsutomu

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9. 佐藤 康史([徳島大学.大学院医歯薬学研究部.医学域.連携研究部門(医学域).寄附講座系(医学域).地域消化器・総合内科学])

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10. (英) Takimoto Rishu

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11. 高山 哲治([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.内科系.消化器内科学])

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12. (英) Kato Junji

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13. (英) Ninomiya Takafumi

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14. (英) Hamada Hirofumi

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15. (英) Niitsu Yoshiro

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(英) Ex vivo large scale generation of human platelets from cord blood CD34+ cells.

(英) In the present investigation, we generated platelets (PLTs) from cord blood (CB) CD34(+) cells using a three-phase culture system. We first cultured 500 CB CD34(+) cells on telomerase gene-transduced human stromal cells (hTERT stroma) in serum-free medium supplemented with stem cell factor (SCF), Flt-3/Flk-2 ligand (FL), and thrombopoietin (TPO) for 14 days. We then transferred the cells to hTERT stroma and cultured for another 14 days with fresh medium containing interleukin-11 (IL-11) in addition to the original cytokine cocktail. Subsequently, we cultured the cells in a liquid culture medium containing SCF, FL, TPO, and IL-11 for another 5 days to recover PLT fractions from the supernatant, which were then gel-filtered to purify the PLTs. The calculated yield of PLTs from 1.0 unit of CB (5 x 10(6) CD34(+) cells) was 1.26 x 10(11) - 1.68 x 10(11) PLTs. These numbers of PLTs are equivalent to 2.5-3.4 units of random donor-derived PLTs or 2/5-6/10 of single-apheresis PLTs. The CB-derived PLTs exhibited features quite similar to those from peripheral blood in morphology, as revealed by electron micrographs, and in function, as revealed by fibrinogen/ADP aggregation, with the appearance of P-selectin and activated glycoprotein IIb-IIIa antigens. Thus, this culture system may be applicable for large-scale generation of PLTs for future clinical use.

1. (英) Antigens, CD34
2. (英) Blood Platelets
3. (英) Cell Culture Techniques
4. 細胞分化(cell differentiation)
5. (英) Cell Lineage
6. 細胞質分裂(cytokinesis)
7. (英) Endothelial Cells
8. (英) Fetal Blood
9. (英) HLA Antigens
10. (英) Humans
11. (英) Megakaryocytes
12. (英) Platelet Membrane Glycoprotein IIb
13. (英) Ploidies
14. (英) Stem Cells
15. (英) Stromal Cells
16. (英) Telomerase

1. (英) Article.Affiliation: Fourth Department of Internal Medicine, Sapporo Medical University, School of Medicine, S1W17, Sapporo, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article