徳島大学 教育・研究者情報データベース(EDB)

1. (英) Yin hua

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2. 原田 永勝

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3. 馬渡 一諭

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4. (英) Sonoko Yasui

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5. 瀬川 博子([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.応用栄養学])

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6. 髙橋 章([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.予防環境栄養学])

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7. 大下 修造

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8. 中屋 豊

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(英) L-DOPA inhibits nitric oxide-dependent vasorelaxation via production of reactive ozygen species in rat aorta

(英) To clarify the underlying mechanisms of L-DOPA induced vasoconstriction in rat aorta. Methods: The effect of L-DOPA on phenylephrine-induced contractile force of blood vessels was examined in vitro using rat aortic ring preparations by isometric tension experiment. Involvement of nitric oxide (NO) in the effect of L-DOPA on vascular smooth muscle was studied by using N(omega)-Nitro-L-arginine (L-NNA), Sodium nitroprusside (SNP) in endothelium-intact and endothelium-denuded aortic rings. L-DOPA potentiated alpha-adrenergic receptor- and depolarization-induced vascular contraction and inhibited acetylcholine-induced vasorelaxation. This effect was diminished by pretreatment of the aortic rings with L-NNA, an inhibitor of NO synthesis, or by removing the endothelium from the ring preparations. In endothelium-denuded rings, L-DOPA inhibited exogenous NO-dependent but not cGMP-mediated vasorelaxation. Increases in cGMP levels in response to an NO donor were attenuated by L-DOPA in cultured rat aortic smooth muscle cells. L-DOPA could not contract rings (without endothelium) pretreated with 3-(5'-hydroxymethyl- 2'-furyl)-1-benzyl indazole (YC-1), an activator of guanylyl cyclase, but SOD (150 U/ml) pretreatment of rings with endothelium inhibited contraction by L-DOPA. These results suggest that L-DOPA inhibits nitric-dependent vasorelaxation on vascular smooth muscle cells via production of reactive oxygen species.

1. (英) Animals
2. (英) Aorta, Thoracic
3. (英) Cells, Cultured
4. (英) Cyclic GMP
5. (英) Drug Synergism
6. (英) Endothelium, Vascular
7. (英) Guanylate Cyclase
8. (英) Levodopa
9. (英) Male
10. (英) Myocytes, Smooth Muscle
11. (英) Nitric Oxide
12. (英) Phenylephrine
13. (英) Rats
14. (英) Rats, Wistar
15. (英) Reactive Oxygen Species
16. (英) Receptors, Cytoplasmic and Nuclear
17. (英) Vasodilation

1. (英) Article.Affiliation: Department of Anesthesiology, Institute of Health Biosciences, the University of Tokushima Graduate School, Tokushima, Japan.

2. (英) Article.PublicationTypeList.PublicationType: In Vitro

3. (英) Article.PublicationTypeList.PublicationType: Journal Article