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著作: Nagasawa H/Hisaeda H/[前川 洋一]/Fujioka H/Ito Y/Aikawa M/Himeno K/gamma delta T cells play a crucial role in the expression of 65,000 MW heat-shock protein in mice immunized with Toxoplasma antigen./[Immunology]

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EID
193210
EOID
667673
Map
0
LastModified
2012年10月26日(金) 11:22:39
Operator
三木 ちひろ
Avail
TRUE
Censor
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Owner
[副研究部長]/[徳島大学.大学院ヘルスバイオサイエンス研究部]
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種別 必須
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Nagasawa H
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Hisaeda H
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 前川 洋一
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Fujioka H
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Ito Y
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Aikawa M
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. (英) Himeno K
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) gamma delta T cells play a crucial role in the expression of 65,000 MW heat-shock protein in mice immunized with Toxoplasma antigen.

副題 任意
要約 任意

(英) Toxoplasma gondii is an obligate intracellular protozoan parasite and cellular immunity plays a crucial role in protection against infection with this pathogen. When mice are immunized with Toxoplasma homogenate, they readily acquire resistance against infection with a lethal dose of a low virulence Beverley strain of T. gondii. We have reported previously that expression of 65,000 MW heat-shock protein (hsp 65) in host macrophages closely correlates with protective potentials of hosts, while this protein is not expressed in Toxoplasma themselves. In this study, we examined the mechanism of expression of hsp 65 in mice immunized with Toxoplasma homogenate. Heat-shock protein was detected in peritoneal macrophages of BALB/c mice immunized 7 days previously by electroblot assay with a specific monoclonal antibody (mAb) for microbial hsp 65. Furthermore, an immunogold ultracytochemistry assay demonstrated that this protein was expressed on the cell surface of peritoneal macrophages in immune mice. This expression was not induced in those of immune athymic nude mice and SCID mice. Treatment of BALB/c mice with anti-Thy-1.2 mAb 1 day before immunization led to an almost complete loss of the expression of hsp 65. To determine the subsets of T cells responsible for induction of this protein, mice were depleted of gamma delta T cells, alpha beta T cells, CD4+ T cells or CD8+ T cells by treating with corresponding antibodies before immunization. From these experiments, gamma delta T cells were shown to be essential for the expression of hsp 65, although CD4+ alpha beta T cells also contributed to some extent. Thus, gamma delta T cells appear to play an important role in protective immunity against infection with T. gondii through mediating the expression of hsp 65 in host macrophages.

キーワード 推奨
  1. (英) Animals
  2. (英) Antigens, Protozoan
  3. (英) Bacterial Proteins
  4. (英) Chaperonins
  5. (英) Female
  6. (英) Heat-Shock Proteins
  7. (英) Immunization
  8. (英) Immunoblotting
  9. 免疫組織化学(immunohistochemistry)
  10. (英) Macrophages
  11. (英) Mice
  12. (英) Mice, Inbred BALB C
  13. (英) Mice, SCID
  14. (英) Receptors, Antigen, T-Cell, gamma-delta
  15. Tリンパ球(T lymphocytes)
  16. (英) Toxoplasma
発行所 推奨
誌名 必須 Immunology(British Society for Immunology)
(pISSN: 0019-2805, eISSN: 1365-2567)
ISSN 任意 0019-2805
ISSN: 0019-2805 (pISSN: 0019-2805, eISSN: 1365-2567)
Title: Immunology
Title(ISO): Immunology
Supplier: British Society for Immunology
Publisher: Wiley Publishing
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 83
必須 3
必須 347 352
都市 任意
年月日 必須 1994年 11月 初日
URL 任意
DOI 任意
PMID 任意 7835958    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
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指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Parasitology and Immunology, School of Medicine, University of Tokushima, Japan.

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  4. (英) OtherID: PMC1415035