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著作: Andersen B. Carsten/[阪上 浩]/Nedachi Taku/Kovacina S. Kristina/Clayberger Carol/Conti Marco/Roth A. Richard/Protein kinase B/Akt is essential for the insulin- but not progesterone-stimulated resumption of meiosis in Xenopus oocytes./[The Biochemical Journal]

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EID
192806
EOID
654021
Map
0
LastModified
2012年8月28日(火) 20:27:14
Operator
大家 隆弘
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TRUE
Censor
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Owner
阪上 浩
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種別 必須
言語 必須 英語
招待 推奨
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共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Andersen B. Carsten
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 阪上 浩([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.代謝栄養学]/[徳島大学.糖尿病臨床・研究開発センター])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Nedachi Taku
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Kovacina S. Kristina
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Clayberger Carol
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Conti Marco
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. (英) Roth A. Richard
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Protein kinase B/Akt is essential for the insulin- but not progesterone-stimulated resumption of meiosis in Xenopus oocytes.

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(英) In the present study, we have characterized the Xenopus Akt expressed in oocytes from the African clawed frog Xenopus laevis and tested whether its activity is required for the insulin- and progesterone-stimulated resumption of meiosis. A cDNA encoding the Xenopus Akt was isolated and sequenced, and its expression in the Xenopus oocyte was confirmed by reverse transcription PCR and Northern blotting. Using phosphospecific antibodies and enzyme assays, a large and rapid activation of the Xenopus Akt was observed upon insulin stimulation of the oocytes. In contrast, progesterone caused a modest activation of this kinase with a slower time course. To test whether the activation of Akt was required in the stimulation of the resumption of meiosis, we have utilized two independent approaches: a functional dominant negative Akt mutant and an inhibitory monoclonal antibody. Both the mutant Akt, as well as the inhibitory monoclonal antibody, completely blocked the insulin-stimulated resumption of meiosis. In contrast, both treatments only partially inhibited (by approx. 30%) the progesterone-stimulated resumption of meiosis when submaximal doses of this hormone were utilized. These data demonstrate a crucial role for Akt in the insulin-stimulated cell cycle progression of Xenopus oocytes, whereas Akt may have an ancillary function in progesterone signalling.

キーワード 推奨
  1. (英) Amino Acid Sequence
  2. (英) Animals
  3. (英) Antibodies, Monoclonal
  4. (英) COS Cells
  5. (英) Dose-Response Relationship, Drug
  6. (英) Female
  7. インスリン(insulin)
  8. (英) Meiosis
  9. (英) Microinjections
  10. (英) Molecular Sequence Data
  11. (英) Oocytes
  12. (英) Phylogeny
  13. (英) Progesterone
  14. (英) Protein-Serine-Threonine Kinases
  15. (英) Proto-Oncogene Proteins
  16. (英) Proto-Oncogene Proteins c-akt
  17. (英) RNA, Messenger
  18. (英) Sequence Alignment
  19. シグナル伝達(signal transduction)
  20. アフリカツメガエル(Xenopus laevis)
発行所 推奨
誌名 必須 The Biochemical Journal([The Biochemical Society])
(pISSN: 0264-6021, eISSN: 1470-8728)
ISSN 任意 0264-6021
ISSN: 0264-6021 (pISSN: 0264-6021, eISSN: 1470-8728)
Title: The Biochemical journal
Title(ISO): Biochem J
Publisher: Portland Press, Ltd.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 369
必須 Pt 2
必須 227 238
都市 任意
年月日 必須 2003年 1月 15日
URL 任意
DOI 任意 10.1042/BJ20021243    (→Scopusで検索)
PMID 任意 12374568    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Division of Reproductive Biology, Department of Gynecology and Obstetrics, Stanford University School of Medicine, Stanford, CA 94305, U.S.A.

  2. (英) Article.DataBankList.DataBank.DataBankName: GENBANK

  3. (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AF317656

  4. (英) Article.PublicationTypeList.PublicationType: Journal Article

  5. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  6. (英) Article.PublicationTypeList.PublicationType: Research Support, U.S. Gov't, P.H.S.

  7. (英) OtherID: PMC1223087