徳島大学 教育・研究者情報データベース(EDB)

1. 三好 圭子([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔生命科学])

   役割	任意
   貢献度	任意
   学籍番号	推奨

2. (英) Nagata Hideya

   役割	任意
   貢献度	任意
   学籍番号	推奨

3. 堀口 大吾([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔生命科学])

   役割	任意
   貢献度	任意
   学籍番号	推奨

4. 阿部 佳織

   役割	任意
   貢献度	任意
   学籍番号	推奨

5. (英) Arie Wahyudi Ivan / (日) IVAN ARIE WAHYUDI / (読) いふぁん あり わひゅでぃ

   役割	任意
   貢献度	任意
   学籍番号	推奨	****

6. 馬場 嘉信

   役割	任意
   貢献度	任意
   学籍番号	推奨

7. (英) Harada Hidemitsu

   役割	任意
   貢献度	任意
   学籍番号	推奨

8. 野間 隆文

   役割	任意
   貢献度	任意
   学籍番号	推奨

(英) BMP2-induced gene profiling in dental epithelial cell line.

(英) Tooth development is regulated by epithelial-mesenchymal interactions and their reciprocal molecular signaling. Bone morphogenetic protein 2 (BMP2) is known as one of the inducers for tooth development. To analyze the molecular mechanisms of BMP2 on ameloblast differentiation (amelogenesis), we performed microarray analyses using rat dental epithelial cell line, HAT-7. After confirming that BMP2 could activate the canonical BMP-Smads signaling in HAT-7 cells, we analyzed the effects of BMP2 on 14,815 gene expressions and profiled them. Seventy-three genes were up-regulated and 28 genes were down-regulated by BMP2 treatment for 24 hours in HAT-7 cells. Functional classification revealed that 18% of up-regulated genes were ECM/adhesion molecules present in the enamel organ. Furthermore, we examined the expression of several differentiation markers in dental epithelial four cell-lineages including inner enamel epithelium (ameloblasts), stratum intermedium, stratum reticulum, and outer enamel epithelium. The results indicated that BMP2 might induce at least two different cell-lineage markers including a BMP antagonist expressed in HAT-7 cells, suggesting that BMP2 could accelerate amelogenesis via BMP signaling.

1. (英) Ameloblasts
2. (英) Amelogenesis
3. (英) Animals
4. (英) Base Sequence
5. (英) Bone Morphogenetic Protein 2
6. (英) Cell Line
7. (英) DNA Primers
8. (英) Down-Regulation
9. (英) Epithelial Cells
10. (英) Gene Expression Profiling
11. (英) Oligonucleotide Array Sequence Analysis
12. (英) Rats
13. (英) Reverse Transcriptase Polymerase Chain Reaction
14. (英) Signal Transduction
15. (英) Smad Proteins
16. (英) Up-Regulation

1. (英) Article.Affiliation: Department of Molecular Biology, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't