徳島大学 教育・研究者情報データベース(EDB)

1. (英) Ruspita Intan / (日) INTAN RUSPITA / (読) いんたん るすぴた

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2. 三好 圭子([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔生命科学])

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3. 武藤 太郎

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4. 阿部 佳織

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5. 堀口 大吾([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔生命科学])

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6. 野間 隆文

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(英) Sp6 downregulation of follistatin gene expression in ameloblasts.

(英) Sp6 is a member of the Sp family of transcription factors that regulate a wide range of cellular functions, such as cell growth and differentiation. Sp6, also called epiprofin, is specifically expressed in tooth germ, limb bud, and hair follicle, but there is little information on its function.To investigate the possible role of Sp6 in tooth development, first we established an Sp6-overproducing clone, CHA9, and analyzed the features of the cell, including cell proliferation and gene expression. The parental cells of CHA9 are the ameloblast-lineage G5 cells that we previously established from rat dental epithelia of lower incisor. Sp6 overproduction accelerated cell proliferation and induced the expression of ameloblastin mRNA, a marker of ameloblast differentiation. Second, we performed genome-wide screening of Sp6 target genes by microarray analysis. Out of a total 20,450 genes, 448 genes were up-regulated and 500 genes were down-regulated by Sp6. We found the expression of follistatin, a BMP antagonist, to be 22.4-fold lower in CHA9 than in control cells. Transfection of the Sp6-antisense construct into CHA9 cells restored follistatin expression back to equivalent levels seen in control cells, indicating that Sp6 regulates follistatin gene expression in ameloblasts.Our findings demonstrate that the follistatin gene is one of the Sp6 target genes in ameloblasts and suggest that Sp6 promotes amelogenesis through inhibition of follistatin gene expression.

1. (英) Ameloblasts
2. (英) Amelogenesis
3. (英) Animals
4. (英) COS Cells
5. (英) Cell Differentiation
6. (英) Cercopithecus aethiops
7. (英) Clone Cells
8. (英) Dental Enamel Proteins
9. (英) Down-Regulation
10. (英) Follistatin
11. (英) Gene Expression Regulation, Developmental
12. (英) Kruppel-Like Transcription Factors
13. (英) Oligonucleotide Array Sequence Analysis
14. (英) Rabbits
15. (英) Rats
16. (英) Transfection

1. (英) Article.Affiliation: Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

4. (英) CommentsCorrections.ErratumIn.RefSource: J Med Invest. 2008 Aug;55(3-4):303