著作: Terai Tomoya/[西村 範行]/Kanda Ikuno/[安井 夏生]/[佐々木 卓也]/JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin./[Molecular Biology of the Cell]
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種別 | 必須 | 学術論文(審査論文) | |||
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言語 | 必須 | 英語 | |||
招待 | 推奨 | ||||
審査 | 推奨 | ||||
カテゴリ | 推奨 | ||||
共著種別 | 推奨 | ||||
学究種別 | 推奨 | ||||
組織 | 推奨 | ||||
著者 | 必須 | ||||
題名 | 必須 |
(英) JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin. |
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副題 | 任意 | ||||
要約 | 任意 |
(英) The dynamic turnover of tight junctions (TJs) is essential for epithelial-mesenchymal transitions and/or mesenchymal-epithelial transitions during epithelial morphogenesis. We previously demonstrated that Rab13 specifically mediates the endocytic recycling of occludin. Here, we identified MICAL-L2 (molecule interacting with CasL-like 2) as a novel Rab13-binding protein. Immunoprecipitation and immunofluorescence microscopy showed that MICAL-L2 specifically bound to the GTP-bound form of Rab13 via its C terminus, which contained a coiled-coil domain, and localized at TJs in epithelial MTD-1A cells. Recycling assay demonstrated that a MICAL-L2 mutant lacking the Rab13-binding domain (MICAL-L2-N) specifically inhibited the endocytic recycling of occludin but not transferrin receptor. Ca2+ switch assay further revealed that MICAL-L2-N as well as Rab13 Q67L inhibited the recruitment of occludin to the plasma membrane, the development of transepithelial electrical resistance, and the formation of a paracellular diffusion barrier. MICAL-L2 was displaced from TJs upon actin depolymerization and was distributed along radiating actin cables and stress fibers in Ca2+-depleted MTD-1A and fibroblastic NIH3T3 cells, respectively. These results suggest that MICAL-L2 mediates the endocytic recycling of occludin and the formation of functional TJs by linking Rab13 to actin cytoskeleton. We rename MICAL-L2 as JRAB (junctional Rab13-binding protein). |
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キーワード | 推奨 |
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発行所 | 推奨 | ||||
誌名 | 必須 |
Molecular Biology of the Cell(The American Society for Cell Biology)
(pISSN: 1059-1524, eISSN: 1939-4586)
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巻 | 必須 | 17 | |||
号 | 必須 | 5 | |||
頁 | 必須 | 2465 2475 | |||
都市 | 任意 | ||||
年月日 | 必須 | 2006年 5月 初日 | |||
URL | 任意 | ||||
DOI | 任意 | 10.1091/mbc.E05-09-0826 (→Scopusで検索) | |||
PMID | 任意 | 16525024 (→Scopusで検索) | |||
NAID | 任意 | ||||
WOS | 任意 | ||||
Scopus | 任意 | ||||
評価値 | 任意 | ||||
被引用数 | 任意 | ||||
指導教員 | 推奨 | ||||
備考 | 任意 |
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