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著作: Terai Tomoya/[西村 範行]/Kanda Ikuno/[安井 夏生]/[佐々木 卓也]/JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin./[Molecular Biology of the Cell]

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EID
189963
EOID
656991
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0
LastModified
2012年9月2日(日) 19:26:53
Operator
大家 隆弘
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Owner
佐々木 卓也
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
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著者 必須
  1. (英) Terai Tomoya
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 西村 範行
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Kanda Ikuno
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    貢献度 任意
    学籍番号 推奨
  4. 安井 夏生
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    貢献度 任意
    学籍番号 推奨
  5. 佐々木 卓也([徳島大学])
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    貢献度 任意
    学籍番号 推奨
題名 必須

(英) JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin.

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要約 任意

(英) The dynamic turnover of tight junctions (TJs) is essential for epithelial-mesenchymal transitions and/or mesenchymal-epithelial transitions during epithelial morphogenesis. We previously demonstrated that Rab13 specifically mediates the endocytic recycling of occludin. Here, we identified MICAL-L2 (molecule interacting with CasL-like 2) as a novel Rab13-binding protein. Immunoprecipitation and immunofluorescence microscopy showed that MICAL-L2 specifically bound to the GTP-bound form of Rab13 via its C terminus, which contained a coiled-coil domain, and localized at TJs in epithelial MTD-1A cells. Recycling assay demonstrated that a MICAL-L2 mutant lacking the Rab13-binding domain (MICAL-L2-N) specifically inhibited the endocytic recycling of occludin but not transferrin receptor. Ca2+ switch assay further revealed that MICAL-L2-N as well as Rab13 Q67L inhibited the recruitment of occludin to the plasma membrane, the development of transepithelial electrical resistance, and the formation of a paracellular diffusion barrier. MICAL-L2 was displaced from TJs upon actin depolymerization and was distributed along radiating actin cables and stress fibers in Ca2+-depleted MTD-1A and fibroblastic NIH3T3 cells, respectively. These results suggest that MICAL-L2 mediates the endocytic recycling of occludin and the formation of functional TJs by linking Rab13 to actin cytoskeleton. We rename MICAL-L2 as JRAB (junctional Rab13-binding protein).

キーワード 推奨
  1. (英) Actin Cytoskeleton
  2. (英) Amino Acid Sequence
  3. (英) Animals
  4. (英) Calcium
  5. (英) Cytoskeletal Proteins
  6. (英) Dogs
  7. (英) Endocytosis
  8. (英) Epithelium
  9. (英) Fibroblasts
  10. (英) Membrane Proteins
  11. (英) Mice
  12. (英) Molecular Sequence Data
  13. (英) Mutation
  14. (英) NIH 3T3 Cells
  15. (英) Protein Structure, Tertiary
  16. (英) Tight Junctions
  17. (英) rab GTP-Binding Proteins
発行所 推奨
誌名 必須 Molecular Biology of the Cell(The American Society for Cell Biology)
(pISSN: 1059-1524, eISSN: 1939-4586)
ISSN 任意 1059-1524
ISSN: 1059-1524 (pISSN: 1059-1524, eISSN: 1939-4586)
Title: Molecular biology of the cell
Title(ISO): Mol Biol Cell
Publisher: American Society for Cell Biology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 17
必須 5
必須 2465 2475
都市 任意
年月日 必須 2006年 5月 初日
URL 任意
DOI 任意 10.1091/mbc.E05-09-0826    (→Scopusで検索)
PMID 任意 16525024    (→Scopusで検索)
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指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Biochemistry, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima 770-8503, Japan.

  2. (英) Article.DataBankList.DataBank.DataBankName: GENBANK

  3. (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB182579

  4. (英) Article.PublicationTypeList.PublicationType: Journal Article

  5. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  6. (英) OtherID: PMC1446078