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著作: Nakatsuji Hiroyoshi/[西村 範行]/Yamamura Rie/Kanayama Hiro-omi/[佐々木 卓也]/Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions./[Molecular and Cellular Biology]

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EID
189894
EOID
664095
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0
LastModified
2012年10月9日(火) 18:27:08
Operator
大家 隆弘
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Owner
佐々木 卓也
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
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共著種別 推奨
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著者 必須
  1. (英) Nakatsuji Hiroyoshi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 西村 範行
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Yamamura Rie
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Kanayama Hiro-omi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. 佐々木 卓也([徳島大学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions.

副題 任意
要約 任意

(英) Tight junctions (TJs) are cell-cell adhesive structures that undergo continuous remodeling. We previously demonstrated that Rab13 and a junctional Rab13-binding protein (JRAB)/molecule interacting with CasL-like 2 (MICAL-L2) localized at TJs and mediated the endocytic recycling of the integral TJ protein occludin and the formation of functional TJs. Here, we investigated how JRAB/MICAL-L2 was targeted to TJs. Using a series of deletion mutants, we found the plasma membrane (PM)-targeting domain within JRAB/MICAL-L2. We then identified actinin-4, which was originally isolated as an actin-binding protein associated with cell motility and cancer invasion/metastasis, as a binding protein for the PM-targeting domain of JRAB/MICAL-L2, using a yeast two-hybrid system. Actinin-4 was colocalized with JRAB/MICAL-L2 at cell-cell junctions and linked JRAB/MICAL-L2 to F-actin. Although actinin-4 bound to JRAB/MICAL-L2 without Rab13, the actinin-4-JRAB/MICAL-L2 interaction was enhanced by Rab13 activation. Depletion of actinin-4 by using small interfering RNA inhibited the recruitment of occludin to TJs during the Ca(2+) switch. During the epithelial polarization after replating, JRAB/MICAL-L2 was recruited from the cytosol to cell-cell junctions. This JRAB/MICAL-L2 recruitment as well as the formation of functional TJs was delayed in actinin-4-depleted cells. These results indicate that actinin-4 is involved in recruiting JRAB/MICAL-L2 to cell-cell junctions and forming functional TJs.

キーワード 推奨
  1. (英) Actinin
  2. (英) Actins
  3. (英) Animals
  4. (英) Base Sequence
  5. (英) Binding Sites
  6. (英) Calcium
  7. (英) Cell Line
  8. (英) Cell Polarity
  9. (英) Cricetinae
  10. (英) Cytoskeletal Proteins
  11. (英) Epithelial Cells
  12. (英) Genetic Vectors
  13. (英) Intercellular Junctions
  14. (英) Membrane Proteins
  15. (英) Mice
  16. (英) Microfilament Proteins
  17. (英) Plasmids
  18. (英) Protein Binding
  19. (英) Protein Structure, Tertiary
  20. (英) RNA, Small Interfering
  21. (英) Recombinant Proteins
  22. (英) Sequence Deletion
  23. (英) Tight Junctions
  24. (英) Transfection
  25. (英) Two-Hybrid System Techniques
  26. (英) rab GTP-Binding Proteins
発行所 推奨
誌名 必須 Molecular and Cellular Biology([アメリカ微生物学会])
(pISSN: 0270-7306, eISSN: 1098-5549)
ISSN 任意 1098-5549
ISSN: 0270-7306 (pISSN: 0270-7306, eISSN: 1098-5549)
Title: Molecular and cellular biology
Title(ISO): Mol Cell Biol
Publisher: American Society for Microbiology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 28
必須 10
必須 3324 3335
都市 任意
年月日 必須 2008年 5月 初日
URL 任意
DOI 任意 10.1128/MCB.00144-08    (→Scopusで検索)
PMID 任意 18332111    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Biochemistry, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8503, Japan.

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  4. (英) OtherID: PMC2423145