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著作: [西村 範行]/[佐々木 卓也]/Cell-Surface Biotinylation to Study Endocytosis and Recycling of Occludin./[Methods in Molecular Biology]

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EID
189887
EOID
665607
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0
LastModified
2012年10月16日(火) 12:59:04
Operator
大家 隆弘
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TRUE
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Owner
佐々木 卓也
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 西村 範行
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 佐々木 卓也([徳島大学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Cell-Surface Biotinylation to Study Endocytosis and Recycling of Occludin.

副題 任意
要約 任意

(英) The dynamic turnover of adherens junctions (AJs) and tight junctions (TJs) is essential for epithelial morphogenesis during normal development and differentiation. Although the endocytic recycling of E-cadherin is characterized and implicated in AJ turnover, the molecular basis for TJ turnover is poorly understood. Occludin and claudins are distinct transmembrane proteins localized to the TJs. Although claudins are an indispensable structural component of TJ strands, depletion of occludin in mice reveals well-developed TJ strands and complex histological abnormalities. To examine the intracellular transport of transmembrane proteins to and from the cell surface, cell-surface biotinylation is a proven powerful method. Using this method, we successfully demonstrated that occludin was endocytosed and recycled back to the cell surface in both fibroblastic baby hamster kidney (BHK) and epithelial MTD-1A cells. The endocytic recycling of occludin as well as the formation of functional TJs was dependent on Rab13 and a junctional Rab13-binding protein (JRAB)/molecule interacting with CasL-like 2 (MICAL-L2). We describe the method to study the intracellular transport of occludin to and from the cell surface in both fibroblastic and epithelial cells.

キーワード 推奨
  1. (英) Animals
  2. (英) Biological Assay
  3. (英) Biotinylation
  4. (英) Cell Line
  5. (英) Cell Line, Tumor
  6. (英) Cell Membrane
  7. (英) Cricetinae
  8. (英) Cytoskeletal Proteins
  9. (英) Endocytosis
  10. (英) Epithelial Cells
  11. (英) Fibroblasts
  12. (英) Humans
  13. (英) Membrane Proteins
  14. (英) Mice
  15. (英) Recombinant Proteins
  16. (英) Tight Junctions
  17. (英) rab GTP-Binding Proteins
発行所 推奨
誌名 必須 Methods in Molecular Biology([Springer])
(pISSN: 1064-3745, eISSN: 1940-6029)
ISSN 任意 1064-3745
ISSN: 1064-3745 (pISSN: 1064-3745, eISSN: 1940-6029)
Title: Methods in molecular biology (Clifton, N.J.)
Title(ISO): Methods Mol Biol
Publisher: Humana Press, Inc.
 (NLM Catalog  (Scopus (Scopus information is found. [need login])
必須 440
必須 ---
必須 89 96
都市 任意
年月日 必須 2008年 2月 初日
URL 任意
DOI 任意 10.1007/978-1-59745-178-9_7    (→Scopusで検索)
PMID 任意 18369939    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Biochemistry, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article