著作: [西村 範行]/[佐々木 卓也]/Identification and Characterization of JRAB/MICAL-L2, a Junctional Rab13-Binding Protein./[Methods in Enzymology]
ヘルプを読む
「著作」(著作(著書,論文,レター,国際会議など))は,研究業績にかかる著作(著書,論文,レター,国際会議など)を登録するテーブルです. (この情報が属するテーブルの詳細な定義を見る)
- 項目名の部分にマウスカーソルを置いて少し待つと,項目の簡単な説明がツールチップ表示されます.
種別 | 必須 | 学術論文(審査論文) | |||
---|---|---|---|---|---|
言語 | 必須 | 英語 | |||
招待 | 推奨 | ||||
審査 | 推奨 | ||||
カテゴリ | 推奨 | ||||
共著種別 | 推奨 | ||||
学究種別 | 推奨 | ||||
組織 | 推奨 |
|
|||
著者 | 必須 | ||||
題名 | 必須 |
(英) Identification and Characterization of JRAB/MICAL-L2, a Junctional Rab13-Binding Protein. |
|||
副題 | 任意 | ||||
要約 | 任意 |
(英) The Rab family small G proteins are localized to distinct subsets of intracellular membranes and play a key role in membrane traffic through the interaction with their specific effector protein(s). Rab13 is identified as a plaque protein at tight junctions (TJs) and has been shown to regulate the assembly of functional TJs in epithelial cells. We have demonstrated that Rab13 mediates the endocytic recycling of integral TJ protein occludin, and identified a junctional Rab13-binding protein (JRAB)/molecule interacting with CasL-like 2 (MICAL-L2) as a Rab13 effector protein using a yeast two-hybrid system. JRAB/MICAL-L2 has a calponin-homology domain in the N-terminus, a LIM domain in the middle, and a coiled-coil domain at the C-terminus, and specifically binds to the GTP-bound form of Rab13 via its C-terminus. It is localized to TJs in epithelial cells and distributed along stress fibers in fibroblasts. In epithelial cells, JRAB/MICAL-L2 as well as Rab13 mediates the endocytic recycling of occludin, but not transferrin receptor, and the formation of functional TJs. This chapter describes the procedures for the isolation of JRAB/MICAL-L2 and the analysis of its functions. |
|||
キーワード | 推奨 |
|
|||
発行所 | 推奨 | ||||
誌名 | 必須 |
Methods in Enzymology([Academic Press])
(pISSN: 0076-6879, eISSN: 1557-7988)
|
|||
巻 | 必須 | 438 | |||
号 | 必須 | --- | |||
頁 | 必須 | 141 153 | |||
都市 | 任意 | ||||
年月日 | 必須 | 2008年 3月 初日 | |||
URL | 任意 | ||||
DOI | 任意 | 10.1016/S0076-6879(07)38010-5 (→Scopusで検索) | |||
PMID | 任意 | 18413246 (→Scopusで検索) | |||
NAID | 任意 | ||||
WOS | 任意 | ||||
Scopus | 任意 | ||||
評価値 | 任意 | ||||
被引用数 | 任意 | ||||
指導教員 | 推奨 | ||||
備考 | 任意 |
|