著作: [宇都 義浩]/Abe Chiaki/Endo Yoshio/[中田 栄司]/[永澤 秀子]/[堀 均]/Development of an in vivo evaluation system of antioxidants for their vascular protective activities using the chick embryo chorioallantoic membrane/ISOTT2008 (2008 International Society on Oxygen Transport to Tissue Conference)
(英) Development of an in vivo evaluation system of antioxidants for their vascular protective activities using the chick embryo chorioallantoic membrane
(英) The blood vessel is one of the most important organs in the oxygen transport, and the drug discovery research of clinical-use antioxidants, which may control various vascular disorders caused by the oxidative stress, is extremely important. In fact, the in vitro evaluation system of antioxidants being mainly used now, however, has a problem of no appreciable in vivo redox status of the antioxidants. We, therefore, tried the development of an in vivo model for the evaluation of antioxidants for their vascular protective activities using the chick embryo having many advantages, such as handy, quick, cheap, and possible high-throughput screening. The topical administration (doses from 150 to 350 g) of 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AAPH), a water-soluble prooxidant, to the chorioallantoic membrane (CAM) of 6-days chick embryos, resulted in their vascular injuries of percentage from 25 to 100% dose dependently, which were correlated with their increased fatal rate of the percentage from 29 to 86%. Artepillin C, a potent natural antioxidant, showed a weak vascular injury (26% at 10 g). These results suggest that immature developing CAM vasculature might have low tolerance to oxidative injury. In the case of 12-days chick embryos, AAPH (doses from 0.3 to 7 mg) induced their vascular injuries (20 to 80%) against the CAM veins and venous capillaries without their fatal damage. Artepillin C, with its dose of up to 30 g, did not show the chick embryo's venous injury, and pre-treatment with artepillin C dose-dependently protected the CAM venous and venous capillary injuries induced by the post-administration of 3 mg of AAPH: 1 g artepillin C decreased 60% of venous injury. These results suggest that artepillin C efficiently protected the AAPH-induced vascular injury of chick embryonic CAM. In conclusion, artepillin C might be a promising clinical-use antioxidant for prevention of vascular disease.
(英) ISOTT2008 (2008 International Society on Oxygen Transport to Tissue Conference)
|年月日||必須||2008年 8月 6日|