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著作: Yamada Mamoru/Talukder Azam Ali/[新田 剛]/Characterization of the ssnA Gene, Which Is Involved in the Decline of Cell Viability at the Beginning of Stationary Phase in Escherichia coli./[Journal of Bacteriology]

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EID
185226
EOID
673720
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0
LastModified
2012年12月25日(火) 16:12:20
Operator
三木 ちひろ
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[センター長]/[徳島大学.疾患プロテオゲノム研究センター]
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨 研究
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Yamada Mamoru
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Talukder Azam Ali
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 新田 剛
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Characterization of the ssnA Gene, Which Is Involved in the Decline of Cell Viability at the Beginning of Stationary Phase in Escherichia coli.

副題 任意
要約 任意

(英) When grown in rich medium, Escherichia coli exhibits a drastic reduction of the number of viable cells at the beginning of stationary phase. The decline of cell viability was retarded by disruption of the ssnA gene, which was identified as a gene subject to RpoS-dependent negative regulation. Moreover, ssnA expression was induced at the time of decline of cell viability at early stationary phase. The viability decline was augmented in the rpoS background, and this augmentation was suppressed by ssnA mutation. Cloning of the ssnA gene in a multicopy plasmid, pBR322, caused small colony formation and slow growth in liquid medium. Cells harboring the ssnA clone showed aberrant morphology that included enlarged and filamentous shapes. The gene product was identified as a 44-kDa soluble protein, but its function could not be deduced by homology searching. From these results, we conclude that ssnA is expressed in response to a phase-specific signal(s) and that its expression level is controlled by RpoS, by a mechanism which may contribute to determination of cell number in the stationary phase.

キーワード 推奨
  1. (英) Amino Acid Sequence
  2. (英) Bacterial Proteins
  3. (英) Base Sequence
  4. 細胞分裂(cell division)
  5. (英) Cloning, Molecular
  6. (英) Culture Media
  7. (英) DNA Primers
  8. (英) DNA, Bacterial
  9. 大腸菌(Escherichia coli)
  10. (英) Escherichia coli Proteins
  11. (英) Gene Expression Regulation, Bacterial
  12. (英) Genes, Bacterial
  13. (英) Molecular Sequence Data
  14. (英) Mutagenesis, Insertional
  15. (英) Mutation
  16. (英) Plasmids
  17. (英) Sigma Factor
発行所 推奨
誌名 必須 Journal of Bacteriology([アメリカ微生物学会])
(pISSN: 0021-9193, eISSN: 1098-5530)
ISSN 任意 0021-9193
ISSN: 0021-9193 (pISSN: 0021-9193, eISSN: 1098-5530)
Title: Journal of bacteriology
Title(ISO): J Bacteriol
Publisher: American Society for Microbiology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 181
必須 6
必須 1838 1846
都市 任意
年月日 必須 1999年 3月 初日
URL 任意
DOI 任意
PMID 任意 10074077    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan. yamada@agr.yamaguchi-u.ac.jp

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  4. (英) OtherID: PMC93583