徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.疾患酵素学研究センター(2007年4月1日〜2016年3月31日/->組織[徳島大学.先端酵素学研究所.次世代酵素学研究領域])

1. 水野 大

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2. (英) Kubo Itsuka

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3. (英) Ide-Kurihara Mikiko

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4. (英) Ichinomiya Tomoko

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5. 木戸 博([徳島大学.先端酵素学研究所.重点研究部門])

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(英) Modified pulmonary surfactant is a potent adjuvant that stimulates the mucosal IgA production in response to the influenza virus antigen

(英) The intranasal administration of influenza hemagglutinin (HA) vaccine with Surfacten, a modified pulmonary surfactant free of antigenic c-type lectins, as a mucosal adjuvant induced the highest protective mucosal immunity in the airway. The intranasal immunization of mice with HA vaccine (0.2 microg)-Surfacten (0.2 microg) selectively induced the neutralizing anti-HA IgA, but not IgG, and conferred nearly maximal protection in the airway, without inducing a systemic response. In contrast, intranasal inoculation of vaccine with 0.2 microg of the potent mucosal adjuvant cholera toxin B* (CT-B*), prepared by adding 0.2% native CT to the B subunit of CT, induced both anti-HA IgA and IgG in the airway and in the serum. The intranasal administration of HA vaccine alone induced a limited amount of mucosal IgA against influenza virus. Although the s.c. administration of HA vaccine prominently induced serum IgG and IgA, Surfacten and CT-B* did not enhance their induction, and the concentrations of Abs leaking into the airways were insufficient to prevent viral multiplication. The intranasal administration of HA-Surfacten stimulated the expression of MHC class II, CD40, and CD86 molecules in the CD11c-positive cells isolated from the nasal mucosa, but not the expression of cells from the lungs or spleens. Lymphocytes isolated from the airway mucosa after intranasal HA-Surfacten immunization prominently induced TGF-beta1 which, compared with inoculation without Surfacten, promoted an Ag-specific mucosal IgA response. Surfacten alone, however, did not induce TGF-beta1. Our observations suggest that Surfacten, by mimicking the natural surfactant, is an effective mucosal adjuvant in the process of airway immunization.

1. (英) Administration, Intranasal
2. (英) Animals
3. (英) Antibodies, Viral
4. (英) Antigen-Presenting Cells
5. (英) Antigens, Viral
6. 細胞質分裂(cytokinesis)
7. (英) Dose-Response Relationship, Immunologic
8. (英) Female
9. (英) Hemagglutinin Glycoproteins, Influenza Virus
10. (英) Immunity, Mucosal
11. (英) Immunoglobulin A, Secretory
12. (英) Immunoglobulin G
13. (英) Immunologic Factors
14. (英) Influenza A virus
15. (英) Influenza Vaccines
16. (英) Mice
17. (英) Mice, Inbred BALB C
18. (英) Nasal Mucosa
19. (英) Neutralization Tests
20. (英) Pulmonary Surfactants
21. (英) Transforming Growth Factor beta
22. (英) Transforming Growth Factor beta1

1. (英) Article.Affiliation: Division of Enzyme Chemistry, Institute for Enzyme Research, University of Tokushima, Tokushima, Japan.

2. (英) Article.PublicationTypeList.PublicationType: In Vitro

3. (英) Article.PublicationTypeList.PublicationType: Journal Article

4. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't