徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座(2004年4月1日〜2015年3月31日)
2. 徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座(2007年4月1日〜)
3. 徳島大学.大学院ヘルスバイオサイエンス研究部.再生修復医歯学部門.顎口腔病態制御学講座(2004年4月1日〜)

1. 森本 景之

   役割	任意
   貢献度	任意
   学籍番号	推奨

2. (英) Ozaki Akiko

   役割	任意
   貢献度	任意
   学籍番号	推奨

3. 岡村 裕彦

   役割	任意
   貢献度	任意
   学籍番号	推奨

4. 吉田 賀弥([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健支援学]/[徳島大学.歯学部.口腔保健学科.口腔保健支援学講座])

   役割	任意
   貢献度	任意
   学籍番号	推奨

5. (英) Amorim Rabelo Bruna

   役割	任意
   貢献度	任意
   学籍番号	推奨

6. (英) Tanaka Hiroaki

   役割	任意
   貢献度	任意
   学籍番号	推奨

7. 北村 清一郎

   役割	任意
   貢献度	任意
   学籍番号	推奨

8. 羽地 達次

   役割	任意
   貢献度	任意
   学籍番号	推奨

(英) Differntial expression of protein phosphatase type 1 isotypes and nucleolin during cell cycle arrest

(英) In the present study, we examined the expression and cytolocalization of protein phosphatase type 1 (PP1) isoforms and nucleolin in human osteoblastic cell line MG63 cells at two boundaries in the cell cycle. We treated MG63 cells with hydroxyurea and nocodazole to arrest the cells at the G(1)/S and G(2)/M boundaries, respectively. As judged from the results of Western blot analysis, PP1 isoforms were expressed differently at each boundary of the cell cycle. Nucleolin was also shown to have a different expression pattern at each boundary. In the hydroxyurea-treated cells, nucleolus-like bodies were bigger in size and decreased in number compared with those in asynchronized cells. However, the subcellular localization of PP1s and nucleolin was not changed. Anti-nucleolin antibody interacted with 110-kDa and 95-kDa proteins present in asynchronized cells and in the cells treated with hydroxyurea. Treatment of the cells with nocodazole decreased the level of the 95-kDa form of nucleolin. In the nocodazole-treated cells, it was impossible to distinguish the distribution of each protein. The phosphorylation status of nucleolin in the cell cycle arrested samples was examined by 2D-IEF-PAGE followed by Western blot analysis. In the case of asynchronized cells or hydroxyurea-treated ones, nucleolin was located at a basic isoelectric point (dephosphorylated status); whereas in the G(2)/M arrest cells, the isoelectric point of nucleolin shifted to an acidic status, indicating that nucleolin was phosphorylated. The present results indicate that PP1 and nucleolin were differently expressed at G(1)/S and G(2)/M boundaries of the cell cycle and acted in a different fashion during cell-cycle progression.

1. (英) Cell Cycle
2. (英) Cells, Cultured
3. (英) Cyclin B
4. (英) Cyclin B1
5. (英) Cyclin D
6. (英) Cyclins
7. (英) Electrophoresis, Gel, Two-Dimensional
8. (英) Humans
9. (英) Hydroxyurea
10. (英) Isoenzymes
11. (英) Nocodazole
12. (英) Phosphoprotein Phosphatases
13. (英) Phosphoproteins
14. (英) Protein Phosphatase 1
15. (英) Protein Phosphatase 2
16. (英) RNA-Binding Proteins

1. (英) Article.Affiliation: Department of Oral and Maxillofacial Anatomy, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan. morimoto@dent.tokushima-u.ac.jp

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

4. (英) MedlineDate: 2007 Jul-Aug