徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座(2004年4月1日〜2015年3月31日)
2. 徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座(2007年4月1日〜)
3. 徳島大学.大学院ヘルスバイオサイエンス研究部.再生修復医歯学部門.顎口腔病態制御学講座(2004年4月1日〜)

1. 森本 景之

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2. (英) Ozaki Akiko

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3. 岡村 裕彦

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4. 吉田 賀弥([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健支援学]/[徳島大学.歯学部.口腔保健学科.口腔保健支援学講座])

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5. 北村 清一郎

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6. 羽地 達次

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(英) Okadaic acid induces tyrosine phosphorylation of IkBa that mediated by PKR pathway in human osteoblastic MG63 cells

(英) Treatment of human osteosarcoma cell line MG 63 cells with okadaic acid stimulated phosphorylation of IkappaBalpha, as judged from the results of Western blot analysis and a lambda protein phosphatase dephosphorylation assay. The stimulated phosphorylation of IkappaBalpha was both time- and dose-dependent. The phosphorylation sites of IkappaBalpha were taken to be tyrosine residues because the anti-phospho-tyrosine antibody bound to the samples immunoprecipitated with the anti-IkappaBalpha antibody. In the cells treated with 100 nM okadaic acid consequential translocation of NF-kappaB p65 from the cytosol to the nucleus occurred. Double-stranded RNA-dependent protein kinase (PKR) is a player in the cellular antiviral response and is involved in transcriptional stimulation through activation of NF-kappaB. We investigated the functional relationship between PKR and IkappaBalpha phosphorylation by constructing MG 63 PKR K/R cells that produced a catalytically inactive mutant PKR. NF-kappaB p65 was detected in the nucleus of these cells, even in the unstimulated cells. Although IkappaBalpha was degraded phosphorylation of eIF-2 alpha, a substrate of PKR, did not occur in the mutant cells treated with okadaic acid. Our results suggest that okadaic acid-induced tyrosine phosphorylation of IkappaBalpha was mediated by PKR kinase activity, thus indicating the involvement of this kinase in the control mechanism governing the activation of NF-kappaB.

1. (英) Cell Line, Tumor
2. (英) Humans
3. (英) I-kappa B Proteins
4. (英) Okadaic Acid
5. (英) Osteoblasts
6. (英) Osteosarcoma
7. (英) Phosphorylation
8. (英) Phosphotyrosine
9. (英) eIF-2 Kinase

1. (英) Article.Affiliation: Department of Oral and Maxillofacial Anatomy, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, Tokushima 770-8504, Japan. morimoto@dent.tokushima-u.ac.jp

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't