著作: [羽地 達次]/Nagano Toshio/Koide S Samuel/Identification and expression of boar sperm proteins that interact with antibodies in serum from an infertile woman/[The Journal of Experimental Zoology]
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| 種別 | 必須 | 学術論文(審査論文) | |||||||||||||||||||||||||||
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| 言語 | 必須 | 英語 | |||||||||||||||||||||||||||
| 招待 | 推奨 | ||||||||||||||||||||||||||||
| 審査 | 推奨 | Peer Review | |||||||||||||||||||||||||||
| カテゴリ | 推奨 | 研究 | |||||||||||||||||||||||||||
| 共著種別 | 推奨 | ||||||||||||||||||||||||||||
| 学究種別 | 推奨 | ||||||||||||||||||||||||||||
| 組織 | 推奨 |
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| 著者 | 必須 |
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| 題名 | 必須 |
(英) Identification and expression of boar sperm proteins that interact with antibodies in serum from an infertile woman |
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| 副題 | 任意 | ||||||||||||||||||||||||||||
| 要約 | 任意 |
(英) Serum designated as IS obtained from a young healthy infertile woman induced a head-to-head agglutination of ejaculated boar sperm. The immunoglobulin G (IgG) prepared from IS localized to the acrosomal region of the sperm head obtained from the corpus and cauda epididymis as determined by an indirect immunofluorescent method. The IgG interacted with a boar sperm protein with an estimated molecular weight of 45-kDa, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoretic (SDS-PAGE) immunoblotting technique. However, the IgG did not interact with proteins extracted from sperm obtained from the testis and caput epididymis or from non-gonadal tissues including liver, kidney, spleen, muscle and serum. The IgG interacted with additional proteins of about 75- and 38-kDa present in the corpus and cauda epididymal fluids but not those in the caput epididymal fluid. The staining intensity of the 75-kDa band was reduced and that of the 38-kDa was nullified with ejaculated seminal plasma proteins. The interacting proteins were adsorbed when chromatographed on Concanavalin A Sepharose column, suggesting that they are glycoproteins. |
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| キーワード | 推奨 |
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| 発行所 | 推奨 | ||||||||||||||||||||||||||||
| 誌名 | 必須 |
The Journal of Experimental Zoology(Harrison, Ross G (Ross Granville)1870-1959/Brooks, William Keith, 1848-1908/Wistar Institute of Anatomy and Biology/American Society of Zoologists)
(pISSN: 0022-104X, eISSN: 1097-010X)
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| 巻 | 必須 | 259 | |||||||||||||||||||||||||||
| 号 | 必須 | 1 | |||||||||||||||||||||||||||
| 頁 | 必須 | 109 116 | |||||||||||||||||||||||||||
| 都市 | 任意 | ||||||||||||||||||||||||||||
| 年月日 | 必須 | 1991年 7月 初日 | |||||||||||||||||||||||||||
| URL | 任意 | ||||||||||||||||||||||||||||
| DOI | 任意 | 10.1002/jez.1402590113 (→Scopusで検索) | |||||||||||||||||||||||||||
| PMID | 任意 | 2072088 (→Scopusで検索) | |||||||||||||||||||||||||||
| CRID | 任意 | ||||||||||||||||||||||||||||
| WOS | 任意 | ||||||||||||||||||||||||||||
| Scopus | 任意 | 2-s2.0-0026197838 | |||||||||||||||||||||||||||
| 評価値 | 任意 | ||||||||||||||||||||||||||||
| 被引用数 | 任意 | ||||||||||||||||||||||||||||
| 指導教員 | 推奨 | ||||||||||||||||||||||||||||
| 備考 | 任意 |
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