徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座(2004年4月1日〜2015年3月31日)

1. 羽地 達次

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   貢献度	任意
   学籍番号	推奨

2. (英) Koide S Samuel

   役割	任意
   貢献度	任意
   学籍番号	推奨

(英) Protein phosphorylation during 5-hydroxytryptamine-induced maturation of Spisula oocytes

(英) Maturation was induced in Spisula oocytes with 5-hydroxytryptamine (5-HT) creatinine sulfate at a final concentration of 5 microM. After 10 and 30 min of treatment, oocytes were homogenized and the cytosolic and particulate fractions were prepared. The fractions were incubated with [gamma-32P]GTP and [gamma-32P]ATP. The phosphorylated proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The radioactivity in the gels was determined by autoradiography. With [gamma-32P]GTP a marked increase in the radiolabeling of proteins with an estimated Mr of 47,000 and 20,000 in the cytosolic and particulate fractions, respectively, was demonstrated with the 5-HT-treated oocytes, whereas no stimulation was demonstrable with the use of [gamma-32P]ATP. A significant increase in GTP-mediated protein phosphorylation occurred within 10 min after 5-HT treatment before the occurrence of germinal vesicle breakdown, suggesting that this post-translation modification of proteins is an early action of the neurotransmitter in the induction of meiotic reinitiation in oocytes.

1. アデノシン三リン酸(adenosine triphosphate)
2. (英) Animals
3. (英) Bivalvia
4. (英) Cytosol
5. (英) Electrophoresis, Polyacrylamide Gel
6. (英) Guanosine Triphosphate
7. 分子量(molecular weight)
8. (英) Oocytes
9. (英) Phosphoproteins
10. リン酸化(phosphorylation)
11. (英) Serotonin

1. (英) Article.PublicationTypeList.PublicationType: Journal Article

2. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

3. (英) Article.PublicationTypeList.PublicationType: Research Support, U.S. Gov't, Non-P.H.S.