著作: Akita Kazumi/[岡村 裕彦]/[吉田 賀弥]/[森本 景之]/Ogawa-Iyehara Hiroaki/[羽地 達次]/Cobalt chloride induces apoptosis and zinc chloride suppresses cobalt-induced apoptosis by bcl-2 expression in human submandibular gland HSG cells/[International Journal of Oncology]
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種別 | 必須 | 学術論文(審査論文) | |||
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言語 | 必須 | 英語 | |||
招待 | 推奨 | ||||
審査 | 推奨 | Peer Review | |||
カテゴリ | 推奨 | 研究 | |||
共著種別 | 推奨 | 国内共著(徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない)) | |||
学究種別 | 推奨 | ||||
組織 | 推奨 |
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著者 | 必須 | ||||
題名 | 必須 |
(英) Cobalt chloride induces apoptosis and zinc chloride suppresses cobalt-induced apoptosis by bcl-2 expression in human submandibular gland HSG cells |
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副題 | 任意 | ||||
要約 | 任意 |
(英) To determine the effects of cobalt chloride on human submandibular gland cells, HSG cells were exposed to various concentrations of cobalt chloride. Cobalt chloride induced cytotoxicity and cell death in HSG cells as determined by phase-contrast microscopy and WST-1 cell viability assay. By using the Hoechst 33342 staining, marked nuclear condensation and fragmentation of chromatin were observed in cobalt chloride-treated cells. Cobalt chloride induced DNA ladder formation in HSG cells in both dose- and time-dependent manner with maximal effect at a concentration of 0.5 mM and 48 h, respectively. Cobalt chloride inhibited the expression of both Bcl-2 protein and mRNA in dose- and time-dependent manner. Zinc chloride recovered the cobalt-suppressed Bcl-2 expression and protected against cobalt-induced apoptosis in HSG cells. Our results show that the pathway of the apoptosis in HSG cells is regulated by cobalt chloride and zinc chloride. Our results also indicate that cobalt-induced apoptotic steps in HSG cells are related to the production of Bcl-2 protein. |
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キーワード | 推奨 |
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発行所 | 推奨 | ||||
誌名 | 必須 |
International Journal of Oncology(International Center for Cancer Research)
(pISSN: 1019-6439, eISSN: 1791-2423)
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巻 | 必須 | 31 | |||
号 | 必須 | 4 | |||
頁 | 必須 | 923 929 | |||
都市 | 任意 | ||||
年月日 | 必須 | 2007年 10月 初日 | |||
URL | 任意 | ||||
DOI | 任意 | 10.3892/ijo.31.4.923 (→Scopusで検索) | |||
PMID | 任意 | 17786326 (→Scopusで検索) | |||
CRID | 任意 | ||||
WOS | 任意 | 000249795300029 | |||
Scopus | 任意 | 2-s2.0-35848933016 | |||
評価値 | 任意 | ||||
被引用数 | 任意 | ||||
指導教員 | 推奨 | ||||
備考 | 任意 |
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