徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.工学部.電気電子工学科.電気電子システム講座
2. 徳島大学.医学部

1. 池原 敏孝([徳島文理大学])

   役割	任意
   貢献度	任意
   学籍番号	推奨

2. 朴 基豪(University of Medicine and Dentistry of New Jersey)

   役割	任意
   貢献度	任意
   学籍番号	推奨

3. 山口 久雄([徳島文理大学])

   役割	任意
   貢献度	任意
   学籍番号	推奨

4. 細川 敬子

   役割	任意
   貢献度	任意
   学籍番号	推奨

5. 吉﨑 和男

   役割	任意
   貢献度	任意
   学籍番号	推奨

6. 宮本 博司([徳島文理大学])

   役割	任意
   貢献度	任意
   学籍番号	推奨

7. (英) Aizawa Katsuo / (日) 會沢 勝夫 / (読) あいざわ かつお

   役割	任意
   貢献度	任意
   学籍番号	推奨

8. 木内 陽介

   役割	任意
   貢献度	任意
   学籍番号	推奨

(英) Effects on Rb⁺(K⁺) Uptake of HeLa Cells in a High K⁺ Medium of Exposure to a Switched 1.7 Tesla Magnetic Field

(英) Effects of a switched, time-varying 1.7 T magnetic field on Rb(+)(K+) uptake by HeLa S3 cells incubated in an isosmotic high K(+) medium were examined. The magnetic flux density was varied intermittently from 0.07-1.7 T at an interval of 3 s. K(+) uptake was activated by replacement of normal medium by high K(+) medium. A membrane-permeable Ca(2+) chelating agent (BAPTA-AM) and Ca(2+)-dependent K(+) channel inhibitors (quinine, charibdotoxin, and iberiotoxin) were found to reduce the Rb(+)(K+) uptake by about 30-40%. Uptake of K(+) that is sensitive to these drugs is possibly mediated by Ca(2+)-dependent K(+) channels. The intermittent magnetic field partly suppress ed the drug-sensitive K(+) uptake by about 30-40% (P < 0.05). To test the mechanism of inhibition by the magnetic fields, intracellular Ca(2+) concentration ([Ca(2+)]c) was measured using Fura 2-AM. When cells were placed in the high K(&plus;) medium, [Ca(2+)]c increased to about 1.4 times the original level, but exposure to the magnetic fields completely suppressed the increase (P < 0.01). Addition of a Ca(2+) ionophore (ionomycin) to the high K(+) medium increased [Ca(2+)]c to the level of control cells, regardless of exposure to the magnetic field. But the inhibition of K(+) uptake by the magnetic fields was not restored by addition of ionomycin. Based on our previous results on magnetic field-induced changes in properties of the cell membrane, these results indicate that exposure to the magnetic fields partly suppresses K(+) influx, which may be mediated by Ca(2+)-dependent K(+) channels. The suppress ion of K(+) fluxes could relate to a change in electric properties of cell surface and an inhibition of Ca(2+) influx mediated by Ca(2+) channels of either the cell plasma membrane or the inner vesicular membrane of intracellular Ca(2+) stores.

1. カルシウム(calcium)
2. (英) Cell Membrane
3. (英) Charybdotoxin
4. (英) Chelating Agents
5. (英) Culture Media
6. (英) Egtazic Acid
7. (英) Electromagnetic Fields
8. 電気生理学(electrophysiology)
9. (英) Environmental Exposure
10. (英) Fluorescent Dyes
11. (英) Fura-2
12. (英) HeLa Cells
13. (英) Humans
14. (英) Intracellular Membranes
15. (英) Ionomycin
16. (英) Ionophores
17. (英) Magnetics
18. (英) Peptides
19. カリウム(potassium)
20. (英) Potassium Channel Blockers
21. (英) Potassium Channels
22. (英) Quinine
23. (英) Rubidium

1. (英) Article.PublicationTypeList.PublicationType: Journal Article

2. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't