徳島大学 教育・研究者情報データベース(EDB)

1. The Forsyth Institute, Department of Immunology

1. 細川 育子([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯科保存学])

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2. 細川 義隆([徳島大学.病院.診療科.歯科.むし歯科(第一保存科)])

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3. (英) Komatsuzawa H

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4. (英) Goncalves RB

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5. (英) Karimbux N

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6. (英) Napimoga MH

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7. 関 誠([三菱ウェルファーマ 株式会社])

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8. (英) Ouhara K

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9. (英) Sugai M

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10. Taubman Martin([The Forsyth Institute, Department of Immunology])

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11. 河井 敬久([The Forsyth Institute, Department of Immunology])

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(英) Innate immune peptide LL-37 displays distinct expression pattern from beta-defensins in inflamed gingival tissue.

(英) Anti-microbial peptides produced from mucosal epithelium appear to play pivotal roles in the host innate immune defence system in the oral cavity. In particular, human beta-defensins (hBDs) and the cathelicidin-type anti-microbial peptide, LL-37, were reported to kill periodontal disease-associated bacteria. In contrast to well-studied hBDs, little is known about the expression profiles of LL-37 in gingival tissue. In this study, the anti-microbial peptides expressed in gingival tissue were analysed using immunohistochemistry and enxyme-linked immunosorbent assay (ELISA). Immunohistochemistry revealed that neutrophils expressed only LL-37, but not hBD-2 or hBD-3, and that such expression was prominent in the inflammatory lesions when compared to healthy gingivae which showed very few or no LL-37 expressing neutrophils. Gingival epithelial cells (GEC), however, expressed all three examined anti-microbial peptides, irrespective of the presence or absence of inflammation. Moreover, as determined by ELISA, the concentration of LL-37 in the gingival tissue homogenates determined was correlated positively with the depth of the gingival crevice. Stimulation with periodontal bacteria in vitro induced both hBD-2 and LL-37 expressions by GEC, whereas peripheral blood neutrophils produced only LL-37 production, but not hBD-2, in response to the bacterial stimulation. These findings suggest that LL-37 displays distinct expression patterns from those of hBDs in gingival tissue.

1. (英) Adult
2. (英) Aged
3. (英) Antigens, Bacterial
4. (英) Antimicrobial Cationic Peptides
5. (英) Bacteria
6. (英) Cells, Cultured
7. (英) Enzyme-Linked Immunosorbent Assay
8. (英) Female
9. (英) Gene Expression Regulation
10. (英) Gingiva
11. (英) Gingivitis
12. (英) Humans
13. (英) Immunoenzyme Techniques
14. (英) Male
15. (英) Middle Aged
16. (英) Neutrophils
17. (英) RNA, Messenger
18. (英) Reverse Transcriptase Polymerase Chain Reaction
19. (英) beta-Defensins

1. (英) Article.Affiliation: Department of Immunology, The Forsyth Institute, Boston, Massachusetts, USA.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) Article.PublicationTypeList.PublicationType: Research Support, N.I.H., Extramural

4. (英) OtherID: PMC1942065