徳島大学 教育・研究者情報データベース(EDB)

Education and Research Database (EDB), Tokushima University

徳島大学ウェブサイトへのリンク

著作: [石田 竜弘]/岡田 百合絵/Kobayashi Tomotaka/[際田 弘志]/Development of pH-sensitive liposomes that efficiently retain encapsulated doxorubicin (DXR) in blood./[International Journal of Pharmaceutics]

ヘルプを読む

「著作」(著作(著書,論文,レター,国際会議など))は,研究業績にかかる著作(著書,論文,レター,国際会議など)を登録するテーブルです. (この情報が属するテーブルの詳細な定義を見る)

  • 項目名の部分にマウスカーソルを置いて少し待つと,項目の簡単な説明がツールチップ表示されます.

この情報をEDB閲覧画面で開く

EID
164166
EOID
983687
Map
0
LastModified
2020年7月31日(金) 14:41:22
Operator
石田 竜弘
Avail
TRUE
Censor
0
Owner
石田 竜弘
Read
継承
Write
継承
Delete
継承
種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 石田 竜弘([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.薬物動態制御学]/[徳島大学.大学院医歯薬学研究部.薬学域.連携研究部門(薬学域).寄附講座系(薬学域).がん細胞と代謝学]/[徳島大学.大学産業院])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Okada Yurie / (日) 岡田 百合絵 / (読) おかだ ゆりえ
    役割 任意
    貢献度 任意
    学籍番号 推奨 ****
  3. (英) Kobayashi Tomotaka
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 際田 弘志
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Development of pH-sensitive liposomes that efficiently retain encapsulated doxorubicin (DXR) in blood.

副題 任意
要約 任意

(英) We have reported that targeted, pH-sensitive sterically stabilized liposomes are able to increase the cytotoxicity of DXR in vitro against B lymphoma cells, but the rate of release of DXR in plasma was too rapid to permit the results to be extended to in vivo applications. The purpose of the study reported here is two-fold. First, to understand the mechanism of the rapid release of DXR from pH-sensitive sterically stabilized liposomes (PSL) in human plasma. Second, to reformulate the above liposomes to improve their drug retention, while retaining their pH sensitivity. The stability of the PSL formulations in human plasma was evaluated by comparing the rate of release of encapsulated DXR with that of HPTS, a water-soluble fluorescent marker. Since DXR, but not HPTS, a water soluble-less membrane permeable fluorescence marker, was rapidly released from liposomes in the presence of plasma, the rapid release of DXR is likely caused by the diffusion of DXR molecules through the lipid bilayer, not by the disruption of the membrane. In order to develop more stable PSL formulations, various molar ratios of the membrane rigidifying lipid, hydrogenated soy HSPC and/or CHOL, were added to the lipid composition and the rate of release of encapsulated solutes and pH-sensitivity were evaluated. The compositions that showed the best drug retention and pH-sensitivity were a mixture of DOPE/HSPC/CHEMS/CHOL/mPEG(2000)-DSPE at a molar ratio of 4:2:2:2:0.3 and DOPE/HSPC/CHEMS/CHOL at a molar ratio of 4:2:2:2. Our formulations, if targeted to internalizing antigens on cancer cells, may increase intracellular drug release rates within acidic compartment, resulting in a further increase in the therapeutic efficacy of targeted anticancer drug-containing liposomes.

キーワード 推奨
  1. (英) Animals
  2. (英) Antibiotics, Antineoplastic
  3. コレステロール(cholesterol)
  4. 拡散(diffusion)
  5. (英) Doxorubicin
  6. (英) Hydrogen-Ion Concentration
  7. (英) Lipids
  8. リポソーム(liposomes)
  9. (英) Male
  10. (英) Mice
  11. 分子量(molecular weight)
  12. (英) Phosphatidylcholines
  13. (英) Phosphatidylethanolamines
  14. プラズマ(plasma)
  15. (英) Polyethylene Glycols
  16. 溶解度(solubility)
  17. (英) Technology, Pharmaceutical
発行所 推奨
誌名 必須 International Journal of Pharmaceutics(Elsevier B.V.)
(pISSN: 0378-5173, eISSN: 1873-3476)
ISSN 任意 0378-5173
ISSN: 0378-5173 (pISSN: 0378-5173, eISSN: 1873-3476)
Title: International journal of pharmaceutics
Title(ISO): Int J Pharm
Publisher: Elsevier B.V.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 309
必須 1-2
必須 94 100
都市 任意
年月日 必須 2006年 2月 初日
URL 任意
DOI 任意 10.1016/j.ijpharm.2005.11.010    (→Scopusで検索)
PMID 任意 16364578    (→Scopusで検索)
CRID 任意
WOS 任意 000235571400013
Scopus 任意 2-s2.0-31144443980
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Pharmacokinetics and Biopharmaceutics, Graduate School of Pharmaceutical Sciences, The University of Tokushima, 1-78-1 Sho-machi, Tokushima 770-8505, Japan. ishida@ph.tokushima-u.ac.jp

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't