著作: [堀尾 哲也]/Basaki, Akemi/Takeoka, Aya/Yamato, Masayuki/Lethal level overexpression of γ-tubulin in fission yeast causes mitotic arrest./[Cell Motility and the Cytoskeleton]
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| 種別 | 必須 | 学術論文(審査論文) | ||||||||||||||||||||||||||||||||||||
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| 言語 | 必須 | 英語 | ||||||||||||||||||||||||||||||||||||
| 招待 | 推奨 | |||||||||||||||||||||||||||||||||||||
| 審査 | 推奨 | Peer Review | ||||||||||||||||||||||||||||||||||||
| カテゴリ | 推奨 | 研究 | ||||||||||||||||||||||||||||||||||||
| 共著種別 | 推奨 | |||||||||||||||||||||||||||||||||||||
| 学究種別 | 推奨 | |||||||||||||||||||||||||||||||||||||
| 組織 | 推奨 |
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| 著者 | 必須 |
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| 題名 | 必須 |
(英) Lethal level overexpression of γ-tubulin in fission yeast causes mitotic arrest. |
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| 副題 | 任意 | |||||||||||||||||||||||||||||||||||||
| 要約 | 任意 |
(英) gamma-Tubulin is a member of the tubulin superfamily and plays essential roles in microtubule nucleation. While the level of other tubulins, alpha- and beta-tubulin, is strictly regulated in higher eukaryotes and overexpression of beta-tubulin is toxic in yeasts, gamma-tubulin can be overexpressed by fivefold in fission yeast without any obvious defect in growth. Extreme overexpression of gamma-tubulin in mammalian cells caused growth arrest; however, the exact level of gamma-tubulin and the critical level of gamma-tubulin necessary for growth defect were undetermined. We have constructed strains that over- or underexpress gamma-tubulin by placing the gamma-tubulin gene under the control of the inducible nmt1 promoter and its variants. Among these, the weakest promoter was able to produce enough gamma-tubulin to support normal growth when its expression was induced. A strain in which the gamma-tubulin gene was placed under the control of the strongest inducible promoter achieved 160-fold overexpression of gamma-tubulin and its growth was suppressed. Normal cytoplasmic microtubules were mostly lost in gamma-tubulin overexpressing cells and gamma-tubulin was accumulated around the periphery of nuclei. Many of the cells were arrested in mitosis. A small fraction of cells did proceed to undergo nuclear division; however, its process looked either significantly deterred or abnormal. Our results presented here suggest that excess gamma-tubulin disrupts the microtubule array and significantly deters the formation of the mitotic spindle, most likely because of random nucleation of microtubules from excess gamma-tubulin in the cytoplasm. |
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| キーワード | 推奨 |
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| 発行所 | 推奨 | |||||||||||||||||||||||||||||||||||||
| 誌名 | 必須 |
Cell Motility and the Cytoskeleton([Wiley-Liss, Inc.])
(pISSN: 0886-1544, eISSN: 1097-0169)
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| 巻 | 必須 | 44 | ||||||||||||||||||||||||||||||||||||
| 号 | 必須 | 4 | ||||||||||||||||||||||||||||||||||||
| 頁 | 必須 | 284 295 | ||||||||||||||||||||||||||||||||||||
| 都市 | 任意 | |||||||||||||||||||||||||||||||||||||
| 年月日 | 必須 | 1999年 12月 初日 | ||||||||||||||||||||||||||||||||||||
| URL | 任意 | |||||||||||||||||||||||||||||||||||||
| DOI | 任意 | 10.1002/(SICI)1097-0169(199912)44:4<284::AID-CM6>3.0.CO;2-I (→Scopusで検索) | ||||||||||||||||||||||||||||||||||||
| PMID | 任意 | 10602257 (→Scopusで検索) | ||||||||||||||||||||||||||||||||||||
| CRID | 任意 | |||||||||||||||||||||||||||||||||||||
| Scopus | 任意 | |||||||||||||||||||||||||||||||||||||
| researchmap | 任意 | |||||||||||||||||||||||||||||||||||||
| 評価値 | 任意 | |||||||||||||||||||||||||||||||||||||
| 被引用数 | 任意 | 16 | ||||||||||||||||||||||||||||||||||||
| 指導教員 | 推奨 | |||||||||||||||||||||||||||||||||||||
| 備考 | 任意 |
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