著作: [堀尾 哲也]/Oakley, R. Berl/Human gamma-tubulin functions in fission yeast./[The Journal of Cell Biology]
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| 種別 | 必須 | 学術論文(審査論文) | ||||||||||||||||||
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| 言語 | 必須 | 英語 | ||||||||||||||||||
| 招待 | 推奨 | |||||||||||||||||||
| 審査 | 推奨 | Peer Review | ||||||||||||||||||
| カテゴリ | 推奨 | 研究 | ||||||||||||||||||
| 共著種別 | 推奨 | |||||||||||||||||||
| 学究種別 | 推奨 | |||||||||||||||||||
| 組織 | 推奨 |
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| 著者 | 必須 |
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| 題名 | 必須 |
(英) Human gamma-tubulin functions in fission yeast. |
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| 副題 | 任意 | |||||||||||||||||||
| 要約 | 任意 |
(英) gamma-Tubulin is a phylogenetically conserved component of microtubule-organizing centers that is essential for viability and microtubule function. To examine the functional conservation of gamma-tubulin, we have tested the ability of human gamma-tubulin to function in the fission yeast Schizosaccharomyces pombe. We have found that expression of a human gamma-tubulin cDNA restores viability and a near-normal growth rate to cells of S. pombe lacking endogenous gamma-tubulin. Immunofluorescence microscopy showed that these cells contained normal mitotic spindles and interphase microtubule arrays, and that human gamma-tubulin, like S. pombe gamma-tubulin, localized to spindle pole bodies, the fungal microtubule-organizing centers. These results demonstrate that human gamma-tubulin functions in fission yeast, and they suggest that in spite of the great morphological differences between the microtubule-organizing centers of humans and fission yeasts, gamma-tubulin is likely to perform the same tasks in both. They suggest, moreover, that the proteins that interact with gamma-tubulin, including, most obviously, microtubule-organizing center proteins, must also be conserved. We have also found that a fivefold overexpression of S. pombe gamma-tubulin causes no reduction in growth rates or alteration of microtubule organization. We hypothesize that the excess gamma-tubulin is maintained in the cytoplasm in a form incapable of nucleating microtubule assembly. Finally, we have found that expression of human gamma-tubulin or overexpression of S. pombe gamma-tubulin causes no significant alteration of resistance to the antimicrotubule agents benomyl, thiabendazole and nocodazole. |
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| キーワード | 推奨 |
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| 発行所 | 推奨 | The Rockefeller University Press | ||||||||||||||||||
| 誌名 | 必須 |
The Journal of Cell Biology(Rockefeller Institute/American Society for Cell Biology)
(pISSN: 0021-9525, eISSN: 1540-8140)
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| 巻 | 必須 | 126 | ||||||||||||||||||
| 号 | 必須 | 6 | ||||||||||||||||||
| 頁 | 必須 | 1465 1473 | ||||||||||||||||||
| 都市 | 任意 | |||||||||||||||||||
| 年月日 | 必須 | 1994年 6月 初日 | ||||||||||||||||||
| URL | 任意 | |||||||||||||||||||
| DOI | 任意 | 10.1083/jcb.126.6.1465 (→Scopusで検索) | ||||||||||||||||||
| PMID | 任意 | 8089179 (→Scopusで検索) | ||||||||||||||||||
| CRID | 任意 | |||||||||||||||||||
| WOS | 任意 | |||||||||||||||||||
| Scopus | 任意 | |||||||||||||||||||
| 評価値 | 任意 | |||||||||||||||||||
| 被引用数 | 任意 | 48 | ||||||||||||||||||
| 指導教員 | 推奨 | |||||||||||||||||||
| 備考 | 任意 |
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