徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.大学院ヘルスバイオサイエンス研究部.発達予防医歯学部門.病態予防医学講座(2004年4月1日〜)
2. 徳島大学.工学部.生物工学科.生物機能工学講座(〜2023年3月31日)

1. 清水 一郎

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2. (英) Ma Yue-Rong

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3. (英) Mizobuchi Yoko

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4. (英) Liu Fei

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5. (英) Miura Tetsuo

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6. (英) Nakai Yoichiro

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7. (英) Yasuda Mitugi

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8. (英) Shiba Masako

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9. (英) Horie Takahiro

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10. (英) Amagaya Sakae

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11. (英) Kawada Norifumi

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12. 堀 均

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13. 伊東 進

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(英) Effects of Sho-saiko-to, a Japanese Herbal Medicine, on Hepatic Fibrosis in Rats

(英) It has been shown that lipid peroxidation is associated with hepatic fibrosis and stellate cell activation. Sho-saiko-to (TJ-9) is an herbal medicine, which is commonly used to treat chronic hepatitis in Japan, although the mechanism by which TJ-9 protects against hepatic fibrosis is not known. As a result, we assayed the preventive and therapeutic effects of TJ-9 on experimental hepatic fibrosis, induced in rats by dimethylnitrosamine (DMN) or pig serum (PS), and on rat stellate cells and hepatocytes in primary culture, and assessed the antioxidative activities and the active components of TJ-9. Male Wistar rats were given a single intraperitoneal injection of 40 mg/kg DMN or 0.5 mL PS twice weekly for 10 weeks. In each model, rats were fed a basal diet throughout, or the same diet, which also contained 1.5% TJ-9, for 2 weeks before treatment or for the last 2 weeks of treatment. TJ-9 suppressed the induction of hepatic fibrosis, increased hepatic retinoids, and reduced the hepatic levels of collagen and malondialdehyde (MDA), a production of lipid peroxidation. Immunohistochemical examination showed that TJ-9 reduced the deposition of type I collagen and the number of alpha-smooth muscle actin (alpha-SMA) positive-stellate cells in the liver and inhibited, not only lipid peroxidation in cultured rat hepatocytes that were undergoing oxidative stress, but also the production of type I collagen, alpha-SMA expression, cell proliferation, and oxidative burst in cultured rat stellate cells. In addition, TJ-9 inhibited Fe2+/adenosine 5'-diphosphate-induced lipid peroxidation in rat liver mitochondria in a dose-dependent manner and showed radical scavenging activity. Among the active components of TJ-9, baicalin and baicalein were found to be mainly responsible for the antioxidative activity. These findings suggest that Sho-saiko-to (TJ-9) functions as a potent antifibrosuppressant by inhibition of lipid peroxidation in hepatocytes and stellate cells in vivo.

1. (英) Animals
2. (英) Antioxidants
3. (英) Blotting, Western
4. 細胞分裂(cell division)
5. (英) Cells, Cultured
6. コラーゲン(collagen)
7. (英) Drugs, Chinese Herbal
8. (英) Hypolipidemic Agents
9. 免疫組織化学(immunohistochemistry)
10. 脂質過酸化(lipid peroxidation)
11. (英) Liver Cirrhosis, Experimental
12. (英) Male
13. (英) Mitochondria, Liver
14. 酸化ストレス(oxidative stress)
15. 酸素消費(oxygen consumption)
16. (英) Rats
17. (英) Rats, Wistar
18. (英) Respiratory Burst
19. (英) Retinoids
20. (英) Superoxides

1. (英) Article.PublicationTypeList.PublicationType: Journal Article