徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.医学部.医学科.病態予防医学講座

1. 東 満美

   役割	任意
   貢献度	任意
   学籍番号	推奨

2. 芳地 一

   役割	任意
   貢献度	任意
   学籍番号	推奨

3. (英) Nishisako Hirotaka / (読) にしさこ ひろたか

   役割	任意
   貢献度	任意
   学籍番号	推奨

4. 石澤 啓介([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.内科系.臨床薬理学])

   役割	任意
   貢献度	任意
   学籍番号	推奨

5. 土屋 浩一郎([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.医薬品機能生化学]/[徳島大学.医学部.医学科.病態情報医学講座])

   役割	任意
   貢献度	任意
   学籍番号	推奨

6. (英) Teraoka Kazuhiko

   役割	任意
   貢献度	任意
   学籍番号	推奨

7. 池原 敏孝([徳島文理大学])

   役割	任意
   貢献度	任意
   学籍番号	推奨

8. 楠見 武徳

   役割	任意
   貢献度	任意
   学籍番号	推奨

9. 水口 和生

   役割	任意
   貢献度	任意
   学籍番号	推奨

(英) Inhibitory action of novel arginine derivative on catecholamine secretion evoked by acetylcholine from cultured bovine adrenal chromaffin cells

(英) A novel product, 4-amino-5-guanidinopentanoic acid 15-[(4-aminobutyl)-3-aminopropylcarbamoyl] pentadecyl ester (Arg-HSA-Spm), was synthesized based on ptilomycalin A, which is one of the extracts from marine sponge. Arg-HSA-Spm contains arginine in its chemical structure. The pharmacological action of Arg-HSA-Spm on catecholamine secretion from cultured bovine adrenal chromaffin cells was examined. Arg-HSA-Spm inhibited catecholamine secretion stimulated by the physiological secretagog acetylcholine. This inhibitory action of Arg-HSA-Spm on catecholamine secretion induced by 10(-4) M acetylcholine was dose-dependent from 10(-8) M to 10(-5) M. In the presence of 3 x 10(-7) M Arg-HSA-Spm, the stimulation of catecholamine secretion observed by increasing acetylcholine up to 10(-3) M did not reach the maximal level observed without Arg-HSA-Spm. Arg-HSA-Spm at 10(-5) M suppressed both the increase in intracellular free Ca2+ level and the influx of 45Ca2+ induced by 10(-4) M acetylcholine. The Arg-HSA-Spm-induced suppression of intracellular free Ca2+ level, the influx of 45Ca2+ and catecholamine secretion were not observed in the presence of extracellular K+ at 56 mM. The results presented in this study suggested that Arg-HSA-Spm may inhibit the influx of extracellular Ca2+ into the cells, probably through its blocking action related to acetylcholine receptors, resulting in the inhibition of catecholamine secretion in adrenal chromaffin cells.

1. (英) Acetylcholine
2. (英) Adrenal Medulla
3. (英) Alkaloids
4. (英) Animals
5. (英) Arginine
6. カルシウム(calcium)
7. (英) Calcium Radioisotopes
8. (英) Carbamates
9. (英) Catecholamines
10. (英) Cattle
11. (英) Cells, Cultured
12. (英) Chromaffin Cells
13. (英) Dose-Response Relationship, Drug
14. (英) Guanidines
15. (英) Spermidine

1. (英) Article.Affiliation: Department of Pharmacy, Tokushima University Hospital, Tokushima, Japan. mamichan@clin.med.tokushima-u.ac.jp

2. (英) Article.PublicationTypeList.PublicationType: Journal Article