徳島大学 教育・研究者情報データベース(EDB)

1. 長谷川 敬展([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔生理学])

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2. (英) Matsuzaki Toshiyuki / (日) 松崎 利行 / (読) まつざき としゆき

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3. (英) Tajika Yuki / (日) 多鹿 友喜 / (読) たじか ゆうき

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4. (英) Ablimit Abduxukur

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5. (英) Suzuki Takeshi / (日) 鈴木 健史 / (読) すずき たけし

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6. (英) Aoki Takeo / (日) 青木 武生 / (読) あおき たけお

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7. (英) Hagiwara Haruo / (日) 萩原 治夫 / (読) はぎわら はるお

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8. (英) Takata Kuniaki / (日) 高田 邦昭 / (読) たかた くにあき

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(英) Differential localization of aquaporin-2 and glucose transporter 4 in polarized MDCK cells.

(英) Membrane water channel aquaporin-2 (AQP2) and glucose transporter 4 (GLUT4) exhibit a common feature in that they are stored in intracellular storage compartments and undergo translocation to the plasma membrane upon hormonal stimulation. We compared the intracellular localization and trafficking of AQP2 and GLUT4 in polarized Madin-Darby canine kidney cells stably transfected with human AQP2 (MDCK-hAQP2) by immunofluorescence microscopy. When expressed in MDCK-hAQP2 cells, GLUT4 and GLUT4-EGFP were predominantly localized in the perinuclear region close to and within the Golgi apparatus, similar to endogenous GLUT4 in adipocytes and myocytes. In addition, GLUT4 was occasionally seen in EEA1-positive early endosomes. AQP2, on the other hand, was sequestered in subapical Rab11-positive vesicles. In the basal state, the intracellular storage site of GLUT4 was distinct from that of AQP2. Forskolin induced translocation of AQP2 from the subapical storage vesicles to the apical plasma membrane, which did not affect GLUT4 localization. When forskolin was washed out, AQP2 was first retrieved to early endosomes from the apical plasma membrane, where it was partly colocalized with GLUT4. AQP2 was then transferred to Rab11-positive storage vesicles. These results show that AQP2 and GLUT4 share a common compartment after retrieval from the plasma membrane, but their storage compartments are distinct from each other in polarized MDCK-hAQP2 cells.

1. (英) Animals
2. (英) Aquaporin 2
3. (英) Cell Line
4. (英) Cell Polarity
5. (英) Dogs
6. (英) Endosomes
7. (英) Glucose Transporter Type 4
8. (英) Humans
9. (英) Kidney
10. (英) Microscopy, Confocal
11. (英) Protein Transport
12. (英) Transfection

1. (英) Article.Affiliation: Department of Anatomy and Cell Biology, Gunma University Graduate School of Medicine, 3-39-22, Showa-machi, Maebashi, Gunma 371-8511, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't