徳島大学 教育・研究者情報データベース(EDB)

1. (英) Yajima Shinya / (日) 矢島 信弥 / (読) やじま しんや

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2. (英) Kubota Makoto / (日) 久保田 眞 / (読) くぼた まこと

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3. (英) Nakakura Takashi / (日) 中倉 敬 / (読) なかくら たかし

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4. 長谷川 敬展([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔分子生理学])

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5. (英) Katagiri Nobuto / (日) 片桐 信人 / (読) かたぎり のぶと

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6. (英) Tomura Hideaki / (日) 戸村 秀明 / (読) とむら ひであき

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7. (英) Sasayama Yuichi / (日) 笹山 雄一 / (読) ささやま ゆういち

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8. (英) Suzuki Masakazu / (日) 鈴木 雅一 / (読) すずきまさかず

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(英) Cloning and expression of vacuolar proton-pumping ATPase subunits in the follicular epithelium of the bullfrog endolymphatic sac.

(英) In an investigation aimed at clarifying the mechanism of crystal dissolution of the calcium carbonate lattice in otoconia (the mineral particles embedded in the otolithic membrane) of the endolymphatic sac (ELS) of the bullfrog, cDNAs encoding the A- and E-subunits of bullfrog vacuolar proton-pumping ATPase (V-ATPase) were cloned and sequenced. The cDNA of the A-subunit consisted of an 11-bp 5'-untranslated region (UTR), a 1,854-bp open reading frame (ORF) encoding a protein comprising 617 amino acids with a calculated molecular mass of 68,168 Da, and a 248-bp 3'-UTR followed by a poly(A) tail. The cDNA of the E-subunit consisted of a 72-bp 5'-UTR, a 681-bp ORF encoding a protein of 226 amino acids with a calculated molecular mass of 26,020 Da, and a 799-bp 3'-UTR followed by a poly(A) tail. Western blot and immunofluorescence analyses using specific anti-peptide antisera against the V-ATPase A- and E-subunits revealed that these subunits were present in the ELS, urinary bladder, skin, testes, and kidneys. In the ELS, positive cells were scattered in the follicular epithelium which, as revealed by electron microscopy, corresponds to the location of mitochondria-rich cells. These findings suggest that V-ATPase, including the A- and E-subunits, exists in mitochondria-rich cells of the ELS, which might be involved in dissolution of the calcium carbonate crystals in the lumen of the ELS.

1. (英) Amino Acid Sequence
2. (英) Animals
3. (英) Base Sequence
4. (英) Blotting, Western
5. (英) Cloning, Molecular
6. (英) DNA, Complementary
7. (英) Endolymphatic Sac
8. (英) Epithelium
9. (英) Fluorescent Antibody Technique
10. (英) Gene Expression Regulation, Enzymologic
11. (英) Molecular Sequence Data
12. (英) Rana catesbeiana
13. (英) Vacuolar Proton-Translocating ATPases

1. (英) Article.Affiliation: Department of Biology, Faculty of Science, Shizuoka University, Japan.

2. (英) Article.DataBankList.DataBank.DataBankName: GENBANK

3. (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB250091

4. (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB250092

5. (英) Article.PublicationTypeList.PublicationType: Journal Article

6. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't