徳島大学 教育・研究者情報データベース(EDB)

1. (英) Kubota Makoto / (日) 久保田 眞 / (読) くぼた まこと

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2. 長谷川 敬展([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔分子生理学])

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3. (英) Nakakura Takashi / (日) 中倉 敬 / (読) なかくら たかし

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4. (英) Tanii Haruna / (日) 谷井 はるな / (読) たにいはるな

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5. (英) Suzuki Masakazu / (日) 鈴木 雅一 / (読) すずきまさかず

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6. (英) Tanaka Shigeyasu / (日) 田中 滋康 / (読) たなかしげやす

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(英) Molecular and cellular characterization of a new aquaporin, AQP-x5, specifically expressed in the small granular glands of Xenopus skin.

(英) A new toad aquaporin (AQP) cDNA was cloned from a cDNA library constructed from the ventral skin of Xenopus laevis. This AQP (Xenopus AQP-x5) consisted of 273 amino acid residues with a high sequence homology to mammalian AQP5. The predicted amino acid sequence contained the two conserved Asn-Pro-Ala motifs found in all major intrinsic protein (MIP) family members and six putative transmembrane domains. The sequence also contained a mercurial-sensitive cysteine and a putative phosphorylation motif site for protein kinase A at Ser-257. The swelling assay using Xenopus oocytes revealed that AQP-x5 facilitated water permeability. Expression of AQP-x5 mRNA was restricted to the skin, brain, lungs and testes. Immunofluorescence and immunoelectron microscopical studies using an anti-peptide antibody (ST-156) against the C-terminal region of the AQP-x5 protein revealed the presence of immunopositive cells in the skin, with the label predominately localized in the apical plasma membrane of the secretory cells of the small granular glands. These glands are unique both in being close to the epidermal layer of the skin and in containing mitochondria-rich cells with vacuolar H+-ATPase dispersed among its secretory cells. Results from immunohistochemical experiments on the mucous or seromucous glands of several other anurans verified this result. We conclude that the presence of AQP-x5 in the apical plasma membrane of the small granular glands suggests its involvement in water secretion from the skins. The physiological roles of the AQP-x5 protein in the small or mucous glands are discussed.

1. (英) Amino Acid Sequence
2. (英) Animals
3. (英) Anura
4. (英) Aquaporin 5
5. (英) Base Sequence
6. (英) Brain
7. (英) Cloning, Molecular
8. (英) Gene Library
9. (英) Lung
10. (英) Male
11. (英) Molecular Sequence Data
12. (英) Oocytes
13. (英) Protein Structure, Tertiary
14. (英) RNA, Messenger
15. (英) Secretory Vesicles
16. (英) Sequence Analysis, Protein
17. (英) Sequence Homology, Amino Acid
18. (英) Skin
19. (英) Testis
20. (英) Water
21. (英) Xenopus Proteins
22. (英) Xenopus laevis

1. (英) Article.Affiliation: Department of Biology, Faculty of Science, Shizuoka University, Ohya 836, Shizuoka 422-8529, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't