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著作: [長谷川 敬展]/谷井 はるな/鈴木 雅一/田中 滋康/Regulation of water absorption in the frog skins by two vasotocin-dependent water-channel aquaporins, AQP-h2 and AQP-h3./[Endocrinology]

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EID
159568
EOID
691936
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LastModified
2013年6月21日(金) 18:38:04
Operator
大家 隆弘
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Owner
長谷川 敬展
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨 研究
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 長谷川 敬展([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔分子生理学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Tanii Haruna / (日) 谷井 はるな / (読) たにい はるな
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Suzuki Masakazu / (日) 鈴木 雅一 / (読) すずき まさかず
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Tanaka Shigeyasu / (日) 田中 滋康 / (読) たなか しげやす
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    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Regulation of water absorption in the frog skins by two vasotocin-dependent water-channel aquaporins, AQP-h2 and AQP-h3.

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(英) A new frog aquaporin (AQP) cDNA was cloned from a cDNA library constructed from the ventral skin of the tree frog Hyla japonica. This AQP (Hyla AQP-h2) consisted of 268 amino acid residues with a high homology to mammalian AQP2. The predicted amino acid sequence contained the two conserved Asn-Pro-Ala motifs found in all the major intrinsic protein family members and the putative six transmembrane domains. The sequence also contained a mercurial compound: cysteine, one potential N-glycosylation site at Asn-124, and a putative phosphorylation site recognized by protein kinase A at Ser-262. In a swelling assay using Xenopus oocytes, AQP-h2 facilitated water permeability, especially in response to cAMP. Expression of AQP-h2 mRNA was restricted to several tissues including the ventral skin, kidney, and urinary bladder; but with immunofluorescence staining using an antipeptide antibody (ST-140) against the AQP-h2 protein, immunopositive cells were found only in the ventral skin and urinary bladder. In the ventral pelvic skin, the label for AQP-h2 was localized in the entire plasma membrane of the granular cells beneath the outmost layer of the skin and in the basolateral membrane of the granular cells in this layer. In response to vasotocin, however, the label for AQP-h2 became more intense in the apical membrane in the granular cells of the outermost layer, similar to the case for the earlier studied AQP-h3, which was specifically expressed in the ventral skin. Taken together, these findings suggest that not only AQP-h3, but also AQP-h2 acts as a regulator of the water balance in this frog.

キーワード 推奨
  1. (英) Amino Acid Sequence
  2. (英) Animals
  3. (英) Antibody Specificity
  4. (英) Anura
  5. (英) Aquaporins
  6. (英) Base Sequence
  7. (英) Biological Transport
  8. (英) Cloning, Molecular
  9. (英) DNA, Complementary
  10. (英) Epidermis
  11. (英) Gene Expression
  12. (英) Molecular Sequence Data
  13. (英) Oocytes
  14. (英) RNA, Messenger
  15. (英) Vasotocin
  16. (英) Water
  17. (英) Water-Electrolyte Balance
  18. (英) Xenopus laevis
発行所 推奨
誌名 必須 Endocrinology([The Endocrine Society])
(pISSN: 0013-7227, eISSN: 1945-7170)
ISSN 任意 0013-7227
ISSN: 0013-7227 (pISSN: 0013-7227, eISSN: 1945-7170)
Title: Endocrinology
Title(ISO): Endocrinology
Publisher: Endocrine Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 144
必須 9
必須 4087 4096
都市 任意
年月日 必須 2003年 9月 初日
URL 任意
DOI 任意 10.1210/en.2003-0418    (→Scopusで検索)
PMID 任意 12933683    (→Scopusで検索)
NAID 任意
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  1. (英) Article.Affiliation: Department of Biology, Faculty of Science, Shizuoka University, Shizuoka 422-8529, Japan.

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't